Analysis of Allelic Diversity and Genetic Relationships Among Cultivated Mangosteen (Garcinia mangostana L.) in Java, Indonesia Using Microsatellite Markers and Morphological Characters
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- 作者：Deden Derajat Matra ; Roedhy Poerwanto ; Edi Santosa ; Sobir…
- 刊名：Tropical Plant Biology
- 出版年：2016
- 出版时间：March 2016
- 年：2016
- 卷：9
- 期：1
- 页码：29-41
- 全文大小：1,434 KB
- 刊物主题：Plant Sciences; Plant Genetics & Genomics; Plant Breeding/Biotechnology; Plant Ecology; Transgenics;
- 出版者：Springer US
- ISSN：1935-9764
- 卷排序：9
文摘
The genetic variation and relationships of the mangosteen (Garcinia mangostana L.) were observed across mangosteen populations in Java, Indonesia using newly identified microsatellite loci and morphological characters. In this study, we developed an improved protocol to isolate microsatellite loci, named Selective Repeats from AFLP Sequence, by using a hybridized membrane. Twenty microsatellite loci were evaluated using 78 individuals from five mangosteen populations, and we successfully amplified four closely related Garcinia species, including G. malaccensis, G. hombroniana, G. celebica, and G. porrecta. Eight loci were monomorphic and the others were polymorphic. Sixty-nine alleles were found, with 3.491 per locus on an average. Genetic diversity (Hʹ) was calculated with an average across loci within population (HʹS) as 0.39, an average loci across many populations (HʹT) as 0.444, and genetic differentiation (FʹST) as 0.147. Furthermore, based on morphological characters, mangosteen individuals from four populations including Leuwiliang, Wanayasa, Puspahiang, and Kaligesing also had morphologically distinct fruit weight, rind weight, and rind thickness among populations. The study also elucidated the dispersal pattern of mangosteen in Java; the source of the genotype mangosteen in Java population was the Wanayasa population. In addition, we found evidence of tetraploidy in mangosteen. These results have potential applications in future breeding, conservation studies, and genetic assessment of mangosteen and their closely related species.KeywordsApomicticGenetic markerPolymorphismPolyploidSSR