Acidobacteria Community Responses to Nitrogen Dose and Form in Chinese Fir Plantations in Southern China

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• 作者：Caixia Liu ; Yuhong Dong ; Lingyu Hou ; Nan Deng ; Ruzhen Jiao
• 关键词：Acidobacteria ; Subgroup ; Ammonium ; Nitrate ; N dose ; Forest soil
• 刊名：Current Microbiology
• 出版年：2017
• 出版时间：March 2017
• 年：2017
• 卷：74
• 期：3
• 页码：396-403
• 全文大小：
• 刊物类别：Biomedical and Life Sciences
• 刊物主题：Microbiology; Biotechnology;
• 出版者：Springer US
• ISSN：1432-0991
• 卷排序：74

文摘

Acidobacteria is a new bacterial group, identified by molecular research, which is widely distributed and has specific ecological functions in forest soil. In this study, we investigated Acidobacteria response to N input, and the effects were related to N form and dose. The experimental design included two N forms (NH₄+-N and NO₃−-N) and five levels of N deposition (0, 20, 40, 60, 80 kg N ha−1) for 2 years. Research into the Acidobacteria community was conducted using 16Sr RNA gene-based high-throughput pyrosequencing methods. Acidobacteria OTUs and N had a negative relationship in 0–60 kg ha−1 year−1; however, at N doses beyond a certain size, nitrogen might promote an increase in Acidobacteria OTUs. The Acidobacteria relative abundance under NH₄+-N treatment was higher than under NO₃−-N treatment. Acidobacteria relative abundance decreased with increasing of NH₄+-N dose, but increased with increasing NO₃−-N dose. Overall, 13 different Acidobacteria subgroups were identified, with Gp1, Gp2, and Gp3 being dominant. Significant differences in Acidobacteria distribution were primarily caused by N input and pH value. The environmental factors of N were all negatively related to Acidobacteria distribution in low N dose treatments (0–20 kg ha−1 year−1), but were positively related in response to N dose treatments (40–80 kg ha−1 year−1).