Study of thiazole orange in aptamer-based dye-displacement assays

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• 作者：Renjun Pei and Milan N. Stojanovic
• 关键词：Aptamers ; Fluorescence assays ; Thiazole orange ; Displacement assays
• 刊名：Analytical and Bioanalytical Chemistry
• 出版年：2008
• 出版时间：February, 2008
• 年：2008
• 卷：390
• 期：4
• 页码：1093-1099
• 全文大小：257.1 KB
• 刊物类别：Chemistry and Materials Science
• 刊物主题：Chemistry
  Analytical Chemistry
  Food Science
  Inorganic Chemistry
  Physical Chemistry
  Monitoring, Environmental Analysis and Environmental Ecotoxicology
  
• 出版者：Springer Berlin / Heidelberg
• ISSN：1618-2650

文摘

We screened a series of RNA and DNA aptamers for their ability to serve in the dye displacement assays in which analytes compete with TO dye. We conclude that, while the performance of the TO dye displacement approach is not always predictable, it is still a simple and sensitive assay to detect binding between RNA aptamers and small molecules. In particular, we describe efficient assays for tobramycin and theophylline, with up to 90% displacement of TO observed, and we describe the first aptameric assay for cAMP.