Precise karyotyping of carrot mitotic chromosomes using multicolour-FISH with repetitive DNA

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• 作者：A. Nowicka ; E. Grzebelus ; D. Grzebelus
• 关键词：Daucus carota repetitive elements ; fluorescence in situ hybridization ; miniature inverted ; repeat transposable elements
• 刊名：Biologia Plantarum
• 出版年：2016
• 出版时间：January 2016
• 年：2016
• 卷：60
• 期：1
• 页码：25-36
• 全文大小：3,894 KB
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• 作者单位：A. Nowicka (1)
  E. Grzebelus (2)
  D. Grzebelus (2)
  
  1. Department of Cell Biology, Franciszek Gorski Institute of Plant Physiology, Polish Academy of Sciences, Niezapominajek 21, PL-30239, Krakow, Poland
  2. Department of Genetics, Plant Breeding and Seed Science, Institute of Plant Biology and Biotechnology, Faculty of Biotechnology and Horticulture, University of Agriculture in Krakow, Al. 29 Listopada 54, PL-31425, Krakow, Poland
  
• 刊物类别：Biomedical and Life Sciences
• 刊物主题：Life Sciences
  Plant Sciences
  
• 出版者：Springer Netherlands
• ISSN：1573-8264

文摘

Carrot (Daucus carota L.) chromosomes are small and uniform in shape and length. Here, mitotic chromosomes were subjected to multicolour fluorescence in situ hybridization (mFISH) with probes derived from conserved plant repetitive DNA (18-25S and 5S rDNA, telomeres), a carrot-specific centromeric repeat (Cent-Dc), carrot-specific repetitive elements (DCREs), and miniature inverted-repeat transposable elements (MITEs). A set of major chromosomal landmarks comprising rDNA and telomeric and centromeric sequences in combination with chromosomal measurements enabled discrimination of carrot chromosomes. In addition, reproducible and unique FISH patterns generated by three carrot genome-specific repeats (DCRE22, DCRE16, and DCRE9) and two transposon families (DcSto and Krak) in combination with telomeric and centromeric reference probes allowed identification of chromosome pairs and construction of detailed carrot karyotypes. Hybridization patterns for DCREs were observed as pericentromeric and interstitial dotted tracks (DCRE22), signals in pericentromeric regions (DCRE16), or scattered signals (DCRE9) along chromosomes similar to those observed for both MITE families. Additional key words Daucus carota repetitive elements fluorescence in situ hybridization miniature inverted-repeat transposable elements