PDX-1 mRNA-induced reprogramming of mouse pancreas-derived mesenchymal stem cells into insulin-producing cells in vitro

详细信息    查看全文

• 作者：Xing Rong Guo ; Xiao Li Wang ; Man Chol Li…
• 关键词：Pancreas ; derived mesenchymal stem cells (pMSCs) ; Reprogramming ; PDX ; 1 mRNA ; Insulin ; producing cells (IPCs)
• 刊名：Clinical and Experimental Medicine
• 出版年：2015
• 出版时间：November 2015
• 年：2015
• 卷：15
• 期：4
• 页码：501-509
• 全文大小：2,598 KB
• 参考文献：1.Lebastchi J, Deng S, Lebastchi AH, et al. Immune therapy and beta-cell death in type 1 diabetes. Diabetes. 2013;62(5):1676-0.PubMedCentral CrossRef PubMed
  2.Hematti P, Kim J, Stein AP, et al. Potential role of mesenchymal stromal cells in pancreatic islet transplantation. Transplant Rev. 2013;27(1):21-.CrossRef
  3.Gershengorn MC, Geras-Raaka E, Hardikar AA, et al. Extra views are better islet cell precursors generated by epithelial-to-mesenchymal transition? Cell Cycle. 2005;4(3):380-.CrossRef PubMed
  4.Eberhardt M, Salmon P, von Mach M-A, et al. Multipotential nestin and Isl-1 positive mesenchymal stem cells isolated from human pancreatic islets. Biochem Biophys Res commun. 2006;345(3):1167-6.CrossRef PubMed
  5.Atouf F, Park CH, Pechhold K, et al. No evidence for mouse pancreatic beta-cell epithelial-mesenchymal transition in vitro. Diabetes. 2007;56(3):699-02.CrossRef PubMed
  6.Chase LG, Ulloa-Montoya F, Kidder BL, et al. Islet-derived fibroblast-like cells are not derived via epithelial-mesenchymal transition from Pdx-1 or insulin-positive cells. Diabetes. 2007;56(1):3-.CrossRef PubMed
  7.Gallo R, Gambelli F, Gava B, et al. Generation and expansion of multipotent mesenchymal progenitor cells from cultured human pancreatic islets. Cell Death Differ. 2007;14(11):1860-1.CrossRef PubMed
  8.Davani B, Ikonomou L, Raaka BM, et al. Human islet-derived precursor cells are mesenchymal stromal cells that differentiate and mature to hormone-expressing cells in vivo. Stem Cells. 2007;25(12):3215-2.CrossRef PubMed
  9.Kim SJ, Choi YS, Ko ES, et al. Glucose-stimulated insulin secretion of various mesenchymal stem cells after insulin-producing cell differentiation. J Biosci Bioeng. 2012;113(6):771-.CrossRef PubMed
  10.Anzalone R, Iacono ML, Loria T, et al. Wharton’s jelly mesenchymal stem cells as candidates for beta cells regeneration: extending the differentiative and immunomodulatory benefits of adult mesenchymal stem cells for the treatment of type 1 diabetes. Stem Cell Rev Rep. 2011;7(2):342-3.CrossRef
  11.Zulewski H, Abraham EJ, Gerlach MJ, et al. Multipotential nestin-positive stem cells isolated from adult pancreatic islets differentiate ex vivo into pancreatic endocrine, exocrine, and hepatic phenotypes. Diabetes. 2001;50(3):521-3.CrossRef PubMed
  12.Zanini C, Bruno S, Mandili G, et al. Differentiation of mesenchymal stem cells derived from pancreatic islets and bone marrow into islet-like cell phenotype. PLoS ONE. 2011;6(12):e28175.PubMedCentral CrossRef PubMed
  13.Cerf ME. Transcription factors regulating ?2-cell function. Eur J Endocrinol. 2006;155(5):671-.CrossRef PubMed
  14.Jafarian A, Taghikhani M, Abroun S, et al. Generation of high-yield insulin producing cells from human bone marrow mesenchymal stem cells. Mol Biol Rep. 2014;41(7):4783-4.CrossRef PubMed
  15.Wang H, Yang Y, Ho G, et al. Programming of human umbilical cord mesenchymal stem cells in vitro to promote pancreatic gene expression. Mol Med Rep. 2013;8(3):769-4.PubMed
  16.Yuan H, Li J, Xin N, et al. Expression of Pdx1 mediates differentiation from mesenchymal stem cells into insulin-producing cells. Mol Biol Rep. 2010;37(8):4023-1.CrossRef PubMed
  17.Leonhardt C, Schwake G, Stogbauer TR, et al. Single-cell mRNA transfection studies: delivery, kinetics and statistics by numbers. Nanomed Nanotechnol Biol Med. 2014;10(4):679-8.CrossRef
  18.Li DS, Yuan YH, Tu HJ, et al. A protocol for islet isolation from mouse pancreas. Nat Protoc. 2009;4(11):1649-2.CrossRef PubMed
  19. Wang XL, Hu P, Guo XR, et al. Reprogramming Human Umbilical Cord Mesenchymal Stem Cells to Islet-like Cells with in vitro synthesized PDX1 mRNA. Cytotherapy. 2014;16(11):1519-7.
  20.Tropepe V, Sibilia M, Ciruna BG, et al. Distinct neural stem cells proliferate in response to EGF and FGF in the developing mouse telencephalon. Dev Biol. 1999;208(1):166-8.CrossRef PubMed
  21.Otonkoski T, Beattie G, Mally M, et al. Nicotinamide is a potent inducer of endocrine differentiation in cultured human fetal pancreatic cells. J Clin Invest. 1993;92(3):1459.PubMedCentral CrossRef PubMed
  22.Zalzman M, Anker-Kitai L, Efrat S. Differentiation of human liver-derived, insulin-producing cells toward the β-cell phenotype. Diabetes. 2005;54(9):2568-5.CrossRef PubMed
  23.Oh SH, Muzzonigro TM, Bae SH, et al. Adult bone marrow-derived cells trans-differentiating into insulin-producing cells for the treatment of type I diabetes. Lab Invest. 2004;84(5):607-7.CrossRef PubMed
  24.Dominici M, Le Blanc K, Mueller I, et al. Minimal criteria for defining multipotent mesenchymal stromal cells. The international society for cellular therapy position statement. Cytotherapy. 2006;8(4):315-.CrossRef PubMed
  25.Moniri MR, Sun XY, Rayat J, et al. TRAIL-engineered pancreas-derived mesenchymal stem cells: characterization and cytotoxic effects on pancreatic cancer cells. Cancer Gene Ther. 2012;19(9):652-.CrossRef PubMed
  26.
• 作者单位：Xing Rong Guo (1) (2)
  Xiao Li Wang (2)
  Man Chol Li (2)
  Ya Hong Yuan (2)
  Yun Chen (2)
  Dan Dan Zou (2)
  Liu Jiao Bian (1)
  Dong Sheng Li (2)
  
  1. College of Life Sciences, Northwest University, Xi’an, 710069, Shanxi, China
  2. Hubei Key Laboratory of Embryonic Stem Cell Research, Taihe Hospital, Hubei University of Medicine, Shiyan, 442000, Hubei, China
  
• 刊物主题：Internal Medicine; Hematology; Oncology;
• 出版者：Springer Milan
• ISSN：1591-9528

文摘

Pancreatic islet transplantation has remained an effective therapy for type 1 diabetes since 2000. Its widespread use has been prohibited by the shortage of suitable donors. It is critical to explore an applicable alternative for β-cell replacement. This study was performed to generate insulin-producing cells (IPCs) from pancreas-derived mesenchymal stem cells (pMSCs). pMSCs were isolated from discarded pancreatic tissue in the filter liquor during islet isolation procedure in mice and ex vivo expanded in culture. IPCs were induced by transfection of pancreas and duodenal transcription factor 1 (PDX-1) mRNA in vitro. Some islet characteristics were identified on pMSC-derived IPCs in mRNA and protein levels. Our results demonstrated that mouse pMSCs can be transdifferentiated into effective glucose-responsive insulin-producing cells through transfecting synthetic modified PDX-1 mRNA in vitro. The study of PDX-1 mRNA-induced pMSC reprogramming may pave the way toward the development of a novel β-cell source for the treatment of diabetes. Keywords Pancreas-derived mesenchymal stem cells (pMSCs) Reprogramming PDX-1 mRNA Insulin-producing cells (IPCs)