HPV16E7-Specific siRNA Inhibits Cell Proliferation in CaSki Cells

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• 作者：Jun-guo Li (1)
  Li Li (2)
  Shui-wen Zhang (1)
  Xiaoguang Wei (3)
  
  1. Reproductive Medicine Center ; General Hospital of Beijing Military Region ; Beijing ; 100700 ; China
  2. Institute of Field Surgery of Daping Hospital ; Third Military Medical University ; Chongqing ; 400042 ; China
  3. Department of Obstetrics and Gynecology ; General Hospital of Beijing Military Region ; Beijing ; 100700 ; China
  
• 关键词：HPV ; siRNA ; Cervical carcinomas ; E7 gene
• 刊名：Cell Biochemistry and Biophysics
• 出版年：2015
• 出版时间：March 2015
• 年：2015
• 卷：71
• 期：2
• 页码：529-534
• 全文大小：1,517 KB
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• 刊物主题：Biochemistry, general; Pharmacology/Toxicology; Biotechnology; Cell Biology; Biophysics and Biological Physics;
• 出版者：Springer US
• ISSN：1559-0283

文摘

High-risk human papilloma virus (HPV) infection is the main cause for the genesis of cervical carcinomas. After infection, E6 and E7 genes of HPV were integrated to the genome of the cervical epithelium. Continued expression of the transforming oncoproteins E6 and E7 not only drives the neoplastic progression in cervical epithelium, but also plays an important role in maintaining the malignant phenotype of cervical cancer cells. The aim of this study is to explore the effects of liposomal transfection of HPV16E7 siRNA on the proliferation of cervical carcinoma cell line CaSki. The siRNA interfering HPV16E7 gene was synthesized and transfected into CaSki cells by liposome to observe the cell morphology changes under microscope. The cell proliferation index was detected by flow cytometry; HPV16E7 mRNA expression was determined by RT-PCR and its protein level was determined by Western blot. After transfection of the CaSki cell by siRNA, cell proliferation was inhibited significantly, and the expression of HPV16E7 mRNA and protein level of HPV16E7 decreased. HPV16E7 siRNA is able to inhibit growth of CaSki cells. HPV16E7 might become a new target for genetic therapy of cervical carcinoma.