Development of a PCR-based assay to differentiate Cervus elaphus sibiricus from Cervus antlers

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• 作者：Young Hwa Kim (1)
  Jae Woong Lee (1)
  Sungwook Chae (1)
  Sang Ho Moon (2)
  Eui Jeong Do (3)
  Seung Eun Oh (3)
  Gui Jun Zhang (4)
  Mi Young Lee (1)
  
  1. KM-Based Herbal Drug Development Group ; Korea Institute of Oriental Medicine ; 1672 Yuseongdae-ro ; Yuseong-gu ; Daejeon ; 305-811 ; Republic of Korea
  2. Division of Food Bioscience ; Konkuk University ; 268 Chungwondae-ro ; Chungju-si ; Chungbuk ; 380-701 ; Republic of Korea
  3. Department of Biological Sciences ; Konkuk University ; 120 Neungdong-ro ; Gwangiin-gu ; Seoul ; 143-701 ; Republic of Korea
  4. School of Chinese Materia Medica ; Beijing University of Chinese Medicine ; No.6 Wang Jing Zhong Huan ; Nan Lu ; Chaoyang District ; Beijing ; 100102 ; China
  
• 关键词：Agarose gel electrophoresis ; Cervus antlers ; Cervus elaphus sibiricus ; Differentiation ; D ; loop region ; Mitochondrial DNA
• 刊名：Journal of the Korean Society for Applied Biological Chemistry
• 出版年：2015
• 出版时间：February 2015
• 年：2015
• 卷：58
• 期：1
• 页码：61-66
• 全文大小：665 KB
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  11. Kim, YH, Kim, ES, Ko, BS, Oh, SE, Ryuk, JA, Chae, SW, Lee, HW, Choi, GY, Seo, DW, Lee, MY (2012) A PCR-based assay for discriminating Cervus and Rangifer (Cervidae) antlers with mitochondrial DNA polymorphisms. J Anim Sci 90: pp. 2075-2083 4421" target="_blank" title="It opens in new window">CrossRef
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• 刊物主题：Applied Microbiology; Bioorganic Chemistry; Biological Techniques;
• 出版者：Springer Netherlands
• ISSN：2234-344X

文摘

Deer antlers (Cervi Parvum Cornu) are reported to possess various pharmacological properties, including anti-infective, anti-arthritic, anti-allergic, and anti-endometriotic properties, and are credited with reversing memory impairment. To determine the global distribution of Cervus subspecies by analyzing antlers, 166 samples of Cervus subspecies antlers were collected from Russia, Canada, China, New Zealand, and Korea. The respective deer subspecies were identified by amplifying the D-loop region of mitochondrial DNA isolated from the antler samples. On the basis of the mitochondrial DNA sequence, a C. elaphus sibiricus-specific primer was developed that differentiates C. e. sibiricus from four C. e. subspecies (C. e. manitobensis, C. e. nelsoni, C. e. canadensis, and C. e. bactrianus), C. elaphus, and C. nippon. This was confirmed using agarose gel electrophoresis. This primer set produced a specific 396-bp fragment that amplified only C. e. sibiricus in antler samples. A 468-bp fragment derived from the cytochrome b gene was used as the internal control to verify the success of amplification in all samples. This method may assist in rapid and effective differentiation between products from Cervus species and other deer products.