Transcriptional profiling reveals molecular basis and novel genetic targets for improved resistance to multiple fermentation inhibitors in Saccharomyces cerevisiae

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• 作者：Yingying Chen ; Jiayuan Sheng ; Tao Jiang ; Joseph Stevens…
• 关键词：Yeast ; Acetic acid ; Furfural ; RNA ; seq ; Transcription factors ; Metabolic engineering
• 刊名：Biotechnology for Biofuels
• 出版年：2016
• 出版时间：December 2016
• 年：2016
• 卷：9
• 期：1
• 全文大小：2,453 KB
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• 作者单位：Yingying Chen (1)
  Jiayuan Sheng (2)
  Tao Jiang (3)
  Joseph Stevens (2)
  Xueyang Feng (2)
  Na Wei (1)
  
  1. Department of Civil and Environmental Engineering and Earth Sciences, University of Notre Dame, 106E Cushing Hall of Engineering, Notre Dame, South Bend, IN, 46556, USA
  2. Department of Biological Systems Engineering, Virginia Polytechnic Institute and State University, Blacksburg, VA, USA
  3. Department of Civil and Environmental Engineering, University of Pittsburgh, Pittsburgh, PA, USA
  
• 刊物类别：Chemistry and Materials Science
• 刊物主题：Biotechnology
  Plant Breeding/Biotechnology
  Renewable and Green Energy
  Environmental Engineering/Biotechnology
  
• 出版者：BioMed Central
• ISSN：1754-6834

文摘

Background Lignocellulosic biomass is a promising source of renewable biofuels. However, pretreatment of lignocellulosic biomass generates fermentation inhibitors that adversely affect the growth of industrial microorganisms such as Saccharomyces cerevisiae and prevent economic production of lignocellulosic biofuels. A critical challenge on developing S. cerevisiae with improved inhibitor resistance lies in incomplete understanding of molecular basis for inhibitor stress response and limited information on effective genetic targets for increasing yeast resistance to mixed fermentation inhibitors. In this study, we applied comparative transcriptomic analysis to determine the molecular basis for acetic acid and/or furfural resistance in S. cerevisiae.