The wheat homolog of putative nucleotide-binding site–leucine-rich repeat resistance gene TaRGA contributes to resistance against powdery mildew

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• 作者：Defu Wang ; Xiaobing Wang ; Yu Mei ; Hansong Dong
• 关键词：Wheat ; Resistance gene ; NBS ; LRR ; Functional identification
• 刊名：Functional & Integrative Genomics
• 出版年：2016
• 出版时间：March 2016
• 年：2016
• 卷：16
• 期：2
• 页码：115-126
• 全文大小：4,457 KB
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• 作者单位：Defu Wang (1) (2)
  Xiaobing Wang (2)
  Yu Mei (2)
  Hansong Dong (2)
  
  1. College of Life Science, Shanxi Agricultural University, Taigu, Shanxi, 030801, China
  2. National Ministry of Education Key Laboratory of Integrated Management of Crop Diseases and Insect Pests, Nanjing Agricultural University, Nanjing, 210095, China
  
• 刊物类别：Biomedical and Life Sciences
• 刊物主题：Life Sciences
  Cell Biology
  Plant Genetics and Genomics
  Microbial Genetics and Genomics
  Biochemistry
  Bioinformatics
  Animal Genetics and Genomics
  
• 出版者：Springer Berlin / Heidelberg
• ISSN：1438-7948

文摘

Powdery mildew, one of the most destructive wheat diseases worldwide, is caused by Blumeria graminis f. sp. tritici (Bgt), a fungal species with a consistently high mutation rate that makes individual resistance (R) genes ineffective. Therefore, effective resistance-related gene cloning is vital for breeding and studying the resistance mechanisms of the disease. In this study, a putative nucleotide-binding site–leucine-rich repeat (NBS-LRR) R gene (TaRGA) was cloned using a homology-based cloning strategy and analyzed for its effect on powdery mildew disease and wheat defense responses. Real-time reverse transcription-PCR (RT-PCR) analyses revealed that a Bgt isolate 15 and salicylic acid stimulation significantly induced TaRGA in the resistant variety. Furthermore, the silencing of TaRGA in powdery mildew-resistant plants increased susceptibility to Bgt15 and prompted conidia propagation at the infection site. However, the expression of TaRGA in leaf segments after single-cell transient expression assay highly increased the defense responses to Bgt15 by enhancing callose deposition and phenolic autofluorogen accumulation at the pathogen invading sites. Meanwhile, the expression of pathogenesis-related genes decreased in the TaRGA-silenced plants and increased in the TaRGA-transient-overexpressing leaf segments. These results implied that the TaRGA gene positively regulates the defense response to powdery mildew disease in wheat.