Mir-351-5p contributes to the establishment of a pro-inflammatory environment in the H9c2 cell line by repressing PTEN expression

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• 作者：Walmir da Silva ; Robson Augusto Souza dos Santos…
• 关键词：Angiotensin II ; Cell culture ; miRNA ; Post ; transcriptional regulation ; PTEN
• 刊名：Molecular and Cellular Biochemistry
• 出版年：2016
• 出版时间：January 2016
• 年：2016
• 卷：411
• 期：1-2
• 页码：363-371
• 全文大小：1,321 KB
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• 作者单位：Walmir da Silva (1)
  Robson Augusto Souza dos Santos (2)
  Karen C. M. Moraes (3)
  
  1. Laboratório de Bioquímica e Biologia Molecular, Instituto de Ciências Exatas e Biológicas, Núcleo de Pesquisa em Biologia, Universidade Federal de Ouro Preto, Ouro Preto, MG, Brazil
  2. Laboratório de Fisiologia, Departmento de Fisiologia e Biofísica, Universidade Federal de Minas Gerais, Belo Horizonte, MG, Brazil
  3. Laboratório de Biologia Molecular, Departamento de Biologia, Instituto de Biociências, Universidade Estadual Paulista “Júlio de Mesquita Filho”, Rio Claro, SP, 13506-900, Brazil
  
• 刊物类别：Biomedical and Life Sciences
• 刊物主题：Life Sciences
  Biochemistry
  Medical Biochemistry
  Oncology
  Cardiology
  
• 出版者：Springer Netherlands
• ISSN：1573-4919

文摘

The activated renin–angiotensin–aldosterone system modulates several metabolic pathways that contribute to left ventricular hypertrophy and heart failure. In this metabolic system, angiotensin II modulates heart morphophysiological changes triggered by a series of inflammatory and pro-inflammatory responses; however, the fine tuning associated with the control of this biochemical pathway remains unknown. Here, we investigated elements involved in the post-transcriptional regulation of the pro-inflammatory environment in the H9c2 cardiac cell line, focusing on miRNA elements that modulate PTEN expression. A cellular model of investigation was established and the miR-315-5p was identified as a novel element targeting PTEN in this cardiac cell line, thereby controlling the protein level. This interconnected pathway contributes to the control of the pro-inflammatory environment in Ang II-treated cells. Keywords Angiotensin II Cell culture miRNA Post-transcriptional regulation PTEN