Nuclear-mitochondrial incompatibility in interorder rhesus monkey–cow embryos derived from somatic cell nuclear transfer

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• 作者：Daekee Kwon ; Ok-Jae Koo ; Min-Jung Kim ; Goo Jang ; Byeong Chun Lee
• 关键词：Blastocysts ; Cow ; Electron transport chain ; Interorder somatic cell nuclear transfer ; Rhesus monkey
• 刊名：Primates
• 出版年：2016
• 出版时间：October 2016
• 年：2016
• 卷：57
• 期：4
• 页码：471-478
• 全文大小：1,166 KB
• 刊物类别：Biomedical and Life Sciences
• 刊物主题：Life Sciences
  Zoology
  Animal Ecology
  Behavioural Sciences
  Evolutionary Biology
  
• 出版者：Springer Japan
• ISSN：1610-7365
• 卷排序：57

文摘

Monkey interorder somatic cell nuclear transfer (iSCNT) using enucleated cow oocytes yielded poor blastocysts development and contradictory results among research groups. Determining the reason for this low blastocyst development is a prerequisite for optimizing iSCNT in rhesus monkeys. The aim of this study was to elucidate nuclear-mitochondrial incompatibility of rhesus monkey-cow iSCNT embryos and its relationship to low blastocyst development. Cytochrome b is a protein of complex III of the electron transport chain (ETC). According to meta-analysis of amino acid sequences, the homology of cytochrome b is 75 % between rhesus monkeys and cattle. To maintain the function of ETC after iSCNT, 4n iSCNT embryos were produced by fusion of non-enucleated cow oocytes and rhesus monkey somatic cells. The blastocyst development rate of 4n iSCNT embryos was higher than that of 2n embryos (P < 0.01). Formation of reactive oxygen species (ROS) is an indirect indicator of ETC activity of cells. The ROS levels of 4n iSCNT embryos was higher than that of 2n embryos (P < 0.01). Collectively, rhesus monkey iSCNT embryos reconstructed with cow oocytes have nuclear-mitochondrial incompatibility due to fundamental species differences between rhesus monkeys and cattle. Nuclear-mitochondrial incompatibility seems to correlate with low ETC activity and extremely low blastocyst development of rhesus monkey-cow iSCNT embryos.