The importance of a sterile rhizosphere when phenotyping for root exudation

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• 作者：René C. P. Kuijken ; Jan F. H. Snel ; Martijn M. Heddes…
• 关键词：Organic acids ; Phenotyping ; Rhizodeposition ; Rhizosphere ; Tomato ; Root exudation
• 刊名：Plant and Soil
• 出版年：2015
• 出版时间：February 2015
• 年：2015
• 卷：387
• 期：1-2
• 页码：131-142
• 全文大小：790 KB
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  15. Hoekenga OA, Maron LG, Pineros MA, Cancado GMA, Shaff J, Kobayashi Y, Ryan PR, Dong B, Delhaize E, Sasaki T, Matsumoto H, Yamamoto Y, Koyama H, Kochian LV (2006) AtALMT1, which encodes a malate transporter, is identified as
• 作者单位：René C. P. Kuijken (1)
  Jan F. H. Snel (1)
  Martijn M. Heddes (1)
  Harro J. Bouwmeester (2)
  Leo F. M. Marcelis (3)
  
  1. Wageningen University and Research Centre, Greenhouse Horticulture, Droevendaalsesteeg 1, 6700 PB, Wageningen, The Netherlands
  2. Laboratory of Plant Physiology, Wageningen University, Droevendaalsesteeg 1, 6700 PB, Wageningen, The Netherlands
  3. Horticulture and Product Physiology, Wageningen University, Droevendaalsesteeg 1, 6700 PB, Wageningen, The Netherlands
  
• 刊物类别：Biomedical and Life Sciences
• 刊物主题：Life Sciences
  Plant Sciences
  Soil Science and Conservation
  Plant Physiology
  Ecology
  
• 出版者：Springer Netherlands
• ISSN：1573-5036

文摘

Background and aims A fast and reliable phenotyping system forms a major bottleneck in root exudation research. Our goal was to develop such a system to quantify genetic and environmental effects on root exudation. Another aim was characterizing non-sterile phenotyping. Methods We developed a system in which plants can be grown with non-sterile shoot environments and sterile rhizospheres. These sterile systems were compared with non-sterile controls. Results In non-sterile rhizospheres exogenous carbon disappeared quickly with a half-life of 2 to 3?h and root exudate concentrations remained below detection limit. In sterile rhizospheres exogenous carbon levels were relatively stable or depleted slower than in non-sterile rhizospheres and organic acid build-up occurred. Tomato (Solanum lycopersicum) could be grown with sterile roots for several months. Conclusions The differences in carbon depletion in sterile and non-sterile rhizospheres was most likely due to the absence of microbial catabolism in sterile rhizospheres. These results prove that using a sterile phenotyping system is essential to study the quantity and composition of root exudates. The sterile system described in this paper eliminates the obscuring effect caused by microbes on exudation levels. It offers a stable, reliable and easy phenotyping method and can be used to investigate genetic and environmental effects on exudation.