Genetic and phenotypic characterizations of Xenorhabdus species (Enterobacteriales: Enterobacteriaceae) isolated from steinernematid nematodes (Rhabditida: Steinernematidae) in Japan

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• 作者：Ryusei Kuwata ; Mutsuhiro Yoshida ; Toyoshi Yoshiga
• 关键词：16S ribosomal RNA gene ; Entomopathogenic ; Mutualism ; Steinernema ; Symbiont
• 刊名：Applied Entomology and Zoology
• 出版年：2017
• 出版时间：February 2017
• 年：2017
• 卷：52
• 期：1
• 页码：125-134
• 全文大小：
• 刊物主题：Entomology; Agriculture; Applied Ecology; Plant Pathology; Zoology; Environmental Management;
• 出版者：Springer Japan
• ISSN：1347-605X
• 卷排序：52

文摘

The bacterial species of the genus Xenorhabdus in the family Enterobacteriaceae have a mutualistic association with steinernematid entomopathogenic nematodes (EPNs), which have been used as biological control agents against soil insect pests. In this study we present the genetic and phenotypic characterizations of the Xenorhabdus species isolated from steinernematid nematodes in Japan. The 18 Japanese Xenorhabdus isolates were classified into five bacterial species based on 16S ribosomal RNA (16S rRNA) gene sequences: Xenorhabdus bovienii, Xenorhabdus hominickii, Xenorhabdus indica, Xenorhabdus ishibashii, and Xenorhabdus japonica. There was no genetic variation between the 16S RNA sequences among the three X. ishibashii isolates, 0–0.1% variation among the five X. hominickii isolates, and 0–0.5% among the eight X. bovienii isolates. Phenotypic characterization demonstrated that representative isolates of the five bacterial species shared common characteristics of the genus Xenorhabdus, and only X. hominickii isolates produced indole. Symbiotic association and co-speciation of Xenorhabdus bacteria with Steinernema nematodes from Japan are discussed.