Evaluation of Castor Oil Samples for Potential Toxin Contamination

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• 作者：Thomas A. McKeon ; Stephanie A. Patfield…
• 关键词：Toxin detection ; Ricinus communis ; Ricin ; Ricinoleic acid ; Lithium grease ; Oil processing
• 刊名：Journal of the American Oil Chemists' Society
• 出版年：2016
• 出版时间：February 2016
• 年：2016
• 卷：93
• 期：2
• 页码：299-301
• 全文大小：407 KB
• 参考文献：1.McKeon TA, He X, Chen GQ, Ahn YJ, Kang ST, Lin JT (2007) The castor plant as a dedicated industrial crop: elucidation of the enzymology of castor oil biosynthesis. In: Hou CT, Shaw JF (eds) Biocatalysis and biotechnology for functional foods and industrial products. AOCS Press and CRC Press, Boca Raton, pp 545–552
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• 作者单位：Thomas A. McKeon (1)
  Stephanie A. Patfield (1)
  Xiaohua He (1)
  
  1. U.S. Department of Agriculture, Agricultural Research Service, Western Regional Research Center, 800 Buchanan St, Albany, CA, 94710, USA
  
• 刊物类别：Chemistry and Materials Science
• 刊物主题：Chemistry
  Industrial Chemistry and Chemical Engineering
  Analytical Chemistry
  Chemistry
  Biotechnology
  Biomaterials
  Agriculture
  
• 出版者：Springer Berlin / Heidelberg
• ISSN：1558-9331

文摘

Castor oil and its derivatives are widely used as a chemical feedstock for production of lubricants and greases, engineering plastics, plasticizers and surfactants. It also has wide application in consumer goods such as lipstick, deodorants and medicinal uses. Due to concerns about the possible presence of the ricin toxin in the oil, we have tested a collection of castor oils processed using different approaches, including cold-pressed, US Pharmaceutical (USP) grade, and neutralized oils. Water soluble proteins were extracted from oil samples into phosphate-buffered saline containing 0.05 % bovine serum albumin (PBSB) and analyzed for potential ricin contamination by ELISA. Our results indicate that only the cold-pressed castor oil contained measurable levels of the toxin, estimated to be 35 ± 13 μg/l. A normal oral dose of castor oil for laxative use is 14 ml, so even cold-pressed castor oil would be well below the toxic level of 1–5 μg/kg body weight. However, the presence of the toxin indicates that other soluble proteins, including allergens, may be present in cold-pressed castor oil. Keywords Toxin detection Ricinus communis Ricin Ricinoleic acid Lithium grease Oil processing