A comparative study of the effects of Pr3+ and La3+ ions on calcium dependent processes in frog cardiac muscle and rat heart mitochondria
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- 作者：S. M. Korotkov ; K. V. Sobol ; I. V. Shemarova ; V. V. Furaev ; A. R. Shumakov…
- 关键词：myocardium ; inotropic effect ; contraction ; rare earth metals ; Pr3+ ; La3+ ; Ca2+ ; mitochondrial respiration ; ΔΨmito ; vertebrates
- 刊名：Biophysics
- 出版年：2016
- 出版时间：September 2016
- 年：2016
- 卷：61
- 期：5
- 页码：733-740
- 全文大小：
- 刊物主题：Biological and Medical Physics, Biophysics;
- 出版者：Pleiades Publishing
- ISSN：1555-6654
- 卷排序：61
文摘
The inotropic effect of Pr3+ and La3+ ions on the heart muscle of frog Rana ridibunda, as well as the influence of the ions on respiration, swelling, and the potential (ΔΨmito) on the inner membrane of Ca2+- loaded rat heart mitochondria, energized by glutamate and malate or succinate in the presence of rotenone were studied. It was found that 2 mM Pr3+ in Ringer’s solution reduces the force of spontaneous contractions and those induced by electrical stimulation in the heart; it had a negative chronotropic effect, decreasing the frequency of spontaneous contractions. Pr3+ and La3+ prevented a decrease in the 2,4-dinitrophenol (DNP)- uncoupled respiration of energized rat heart mitochondria, swelling of these organelles in salt media, and a reduction in ΔΨmito on the inner mitochondrial membrane that were induced by Ca2+ ions. Retardation by Pr3+ and La3+ ions of these calcium-induced effects may suggest that in the inner mitochondrial membrane these metals inhibit the opening of the mitochondrial permeability transition pore caused by Ca2+ overload of mitochondria. The data we obtained are important for a better understanding of the mechanisms of the damaging action of rare-earth elements on Ca2+-dependent processes in the vertebrate myocardium.