Role of Nanostructures for Anti-proliferation of Bacteria and Their Quantitative Study Validated by Statistical Analysis
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- 作者：Rizwan Wahab (1)
Farheen Khan (2) - 关键词：Analytical methods ; Spectrophotometry ; Nanorods ; Nanospheres ; Nanonuts ; E. coli
- 刊名：Journal of Pharmaceutical Innovation
- 出版年：2014
- 出版时间：December 2014
- 年：2014
- 卷：9
- 期：4
- 页码：282-290
- 全文大小：952 KB
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21. Wahab R, Kim YS, Shin HS. Fabrication, characterization and growth mechanism of heterostructured zinc oxide nanostructures via solution method. 2011; 11(3): 334- - 作者单位：Rizwan Wahab (1)
Farheen Khan (2)
1. College of Science, Department of Zoology, King Saud University, Riyadh, 11451, Saudi Arabia
2. Department of Chemistry, Aligarh Muslim University, Aligarh, UP, 202002, India - ISSN：1939-8042
文摘
The simple and sensitive UV–visible spectrophotometric analytical methods have been adopted for the determination and validation of ZnO nanostructures (nanorods (NRs), nanosphere (NSs), and nanonuts (NNTs)), concentration, which are used to control the bacterial growth of Escherichia coli. This method is based on processing conditions of nanostructures of ZnO using precursors zinc acetate dihydrate (Zn(AC2)2·2H2O), zinc nitrate hexahydrate (Zn(NO3)2·6H2O), sodium hydroxide, hexamethylenetetramine (HMT), methanol, etc. The optical density (OD) of the resulting solution of ZnO nanostructures with E. coli bacteria were measured at 600?nm against the reagent blank, prepared under the same conditions. The use of statistical analysis for evaluation of the resulting solution (ZnO-NRs, ZnO-NSs, and ZnO-NNTs with E. coli) was optimized and validated by various operational parameters. Beer’s law was followed (Concentration range from 0.25-.0?μg?ml?) with apparent molar absorptivity of 4.38?×-02?l?mol??cm? for ZnO-NRs, 2.70?×-02?l?mol??cm? for ZnO-NSs, and 3.10?×-02?l?mol??cm? for ZnO-NNTs, respectively. The calibration curve shows linearity over the concentration range of 0.25-.50?μg?ml? for ZnO-NRs and 0.25-.0?μg?ml? for ZnO-NSs and ZnO-NNTs. Detection limit (LOD) and quantitation limit (LOQ) were found to be 0.022:0.068?μg?ml? for ZnO-NRs, 0.028:0.087?μg?ml? for ZnO-NSs, and 0.044:0.137?μg?ml? for ZnO-NNTs analyzed by spectrophotometric method, respectively.