Induced expression of Serratia marcescens ribonuclease III gene in transgenic Nicotiana tabacum L. cv. SR1 tobacco plants

详细信息    查看全文

• 作者：I. V. Zhirnov ; E. A. Trifonova ; A. V. Romanova…
• 关键词：transgenic plants ; Serratia marcescens RNase III ; induced expression ; tobacco mosaic virus
• 刊名：Russian Journal of Genetics
• 出版年：2016
• 出版时间：November 2016
• 年：2016
• 卷：52
• 期：11
• 页码：1137-1141
• 全文大小：
• 刊物类别：Biomedical and Life Sciences
• 刊物主题：Human Genetics; Animal Genetics and Genomics; Microbial Genetics and Genomics;
• 出版者：Pleiades Publishing
• ISSN：1608-3369
• 卷排序：52

文摘

Transgenic Nicotiana tabacum L. cv. SR1 plants, characterized by an increase in the level of dsRNA-specific hydrolytic activity after induction by wounding, were obtained. The Solanum lycopersicum anionic peroxidase gene promoter (new for plant genetic engineering) was for the first time used for the induced expression of the target Serratia marcescens RNase III gene. Upon infection with the tobacco mosaic virus (TMV), the transgenic plants of the obtained lines did not differ significantly from the control group in the level of TMV capsid protein accumulation. In general, no delay in the development of the infection symptoms was observed in transgenic plants as compared with the control group. The obtained transgenic plants represent a new model for the study of the biological role of endoribonucleases from the RNase III family, including in molecular mechanisms of resistance to pathogens.