Prevalence and molecular identification of Chlamydia abortus in commercial dairy goat farms in a hot region in Mexico

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• 作者：Eleuterio Campos-Hernández (1) (2)
  Juan Carlos Vázquez-Chagoyán (1)
  Abdelfattah Z. M. Salem (1)
  Jorge Antonio Saltijeral-Oaxaca (3)
  Cristina Escalante-Ochoa (4)
  Sandra M. López-Heydeck (1)
  Roberto Montes de Oca-Jiménez (1)
  
• 关键词：Prevalence ; Chlamydia abortus ; Isolation ; PCR
• 刊名：Tropical Animal Health and Production
• 出版年：2014
• 出版时间：August 2014
• 年：2014
• 卷：46
• 期：6
• 页码：919-924
• 全文大小：164 KB
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• 作者单位：Eleuterio Campos-Hernández (1) (2)
  Juan Carlos Vázquez-Chagoyán (1)
  Abdelfattah Z. M. Salem (1)
  Jorge Antonio Saltijeral-Oaxaca (3)
  Cristina Escalante-Ochoa (4)
  Sandra M. López-Heydeck (1)
  Roberto Montes de Oca-Jiménez (1)
  
  1. Facultad de Medicina Veterinaria y Zootecnia, Universidad Autónoma del Estado de México, Estado de México, México
  2. Laboratorio de Epidemiología y Zoonosis, Instituto de Investigación Científica área Ciencias Naturales, UAGro, Chilpancingo, Mexico
  3. Departamento de Producción Agrícola y Animal, UAM-X, Mexico, Mexico
  4. Facultad de Medicina Veterinaria y Zootecnia, UNAM, Mexico, Mexico
  
• ISSN：1573-7438

文摘

The aim of this study was to determine the seroprevalence and presence of Chlamydia abortus in Saanen breed female goats from commercial dairy goat farms under intensive production in the municipality of Guanajuato, Mexico. Sera were collected to determine the prevalence of anti-C. abortus IgG antibodies using recombinant enzyme-linked immunosorbent assay (rELISA) and cell culture. Polymerase chain reaction (PCR) was used to prove the presence of the pathogen in swab samples collected from the vagina and rectum of selected animals. Additionally, foetal tissue samples from a sudden abortion were collected. C. abortus prevalence in female goats of commercial milking farms sampled in Guanajuato, Mexico, was 4.87?% (n--46). Seropositive animals were found in six out of nine (66.6?%) dairy goat farms sampled, and prevalence among animals in individual farms ranged between 3.44 and 13.51?%. C. abortus was detected using PCR in spleen tissue from the aborted foetus. PCR-based detection, as well as isolation from vaginal and rectal swabs, was not possible in the present study. Isolation through cell culture was also unsuccessful from aborted foetal tissue samples. In conclusion, the results from rELISA and PCR show that C. abortus is present in dairy goat farms in the state of Guanajuato, Mexico.