Paenibacillus chinensis sp. nov., isolated from maize (Zea mays L.) seeds

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• 作者：Yang Liu ; Ran Zhao ; Ronghuan Wang ; Su Yao ; Lei Zhai ; Xin Zhang…
• 关键词：Paenibacillus chinensis sp. nov. ; Maize ; 16S rRNA gene ; Polyphasic taxonomy
• 刊名：Antonie van Leeuwenhoek
• 出版年：2016
• 出版时间：February 2016
• 年：2016
• 卷：109
• 期：2
• 页码：207-213
• 全文大小：435 KB
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• 作者单位：Yang Liu (1) (2)
  Ran Zhao (2)
  Ronghuan Wang (1)
  Su Yao (2)
  Lei Zhai (2)
  Xin Zhang (2)
  Chuanyong Chen (1)
  Yanhua Cao (2)
  Tianjun Xu (1)
  Yuanyuan Ge (2)
  Jiuran Zhao (1)
  Chi Cheng (2)
  
  1. Maize Research Center, Beijing Academy of Agriculture and Forestry Sciences, Beijing, 100097, People’s Republic of China
  2. China Center of Industrial Culture Collection (CICC), China National Research Institute of Food and Fermentation Industries, Beijing, 100015, People’s Republic of China
  
• 刊物类别：Biomedical and Life Sciences
• 刊物主题：Life Sciences
  Microbiology
  Medical Microbiology
  Plant Sciences
  Soil Science and Conservation
  
• 出版者：Springer Netherlands
• ISSN：1572-9699

文摘

Four Gram-stain positive bacterial strains, designated as 4R1T, 4R9, 4L13 and 4L18, isolated from seeds of hybrid maize (Zea mays L., Jingke 968), were investigated using a polyphasic taxonomic approach. The cells were found to be facultatively aerobic, motile, spore-forming and rod-shaped. Phylogenetic analysis based on 16S rRNA gene sequences showed that the isolates should be recognised as a species of the genus Paenibacillus, with two close neighbours being Paenibacillus nicotianae YIM h-19T (98.41 % similarity) and Paenibacillus hordei RH-N24T (98.37 %). The DNA G+C content of strain 4R1T was determined to be 51.6 mol %. Its polar lipid profile was found to consist of diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine and an unidentified lipid. The predominant respiratory quinone was identified as MK-7 and the major fatty acids were found to be anteiso-C_15:0, anteiso-C_12:0, anteiso-C_13:0 and anteiso-C_11:0. Strains 4R1T, 4R9, 4L13 and 4L18 were clearly distinguished from the reference type strains using phylogenetic analysis, DNA–DNA hybridization and a range of physiological and biochemical characteristics. It is evident from the genotypic and phenotypic data that strains 4R1T, 4R9, 4L13 and 4L18 represent a novel species of the genus Paenibacillus, for which the name Paenibacillus chinensis sp. nov. is proposed. The type strain is 4R1T (=KCTC 33672T = CICC 23864T). Keywords Paenibacillus chinensis sp. nov. Maize 16S rRNA gene Polyphasic taxonomy