Morphological and molecular characterization of yellow oyster mushroom, Pleurotus citrinopileatus, hybrids obtained by interspecies mating

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• 作者：A. G. Rosnina ; Yee Shin Tan…
• 关键词：Pleurotaceae ; Biological efficiency ; Sporophore ; Monokaryon ; Dikaryon
• 刊名：World Journal of Microbiology & Biotechnology
• 出版年：2016
• 出版时间：February 2016
• 年：2016
• 卷：32
• 期：2
• 全文大小：1,188 KB
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• 作者单位：A. G. Rosnina (1) (2)
  Yee Shin Tan (1)
  Noorlidah Abdullah (1)
  S. Vikineswary (1)
  
  1. Mushroom Research Centre, Institute of Biological Sciences, Faculty of Science, University of Malaya, 50603, Kuala Lumpur, Malaysia
  2. Department of Agroecotechnology of Agriculture Faculty, University of Malikussaleh, Lhokseumawe, Aceh, Indonesia
  
• 刊物类别：Chemistry and Materials Science
• 刊物主题：Chemistry
  Applied Microbiology
  Biotechnology
  Biochemistry
  Environmental Biotechnology
  Microbiology
  
• 出版者：Springer Netherlands
• ISSN：1573-0972

文摘

Pleurotus citrinopileatus (yellow oyster mushroom) has an attractive shape and yellow colour but the fragile texture complicates packaging, and its strong aroma is unappealing to consumers. This study aimed to improve the characteristics and yield of P. citrinopileatus by interspecies mating between monokaryotic cultures of P. citrinopileatus and P. pulmonarius. Ten monokaryon cultures of the parental lines were crossed in all combinations to obtain hybrids. Eleven compatible mating pairs were obtained and cultivated to observe their sporophore morphology and yield. The selected hybrid, i.e. P1xC9, was beige in colour while hybrid P3xC8 was yellow in colour. Their sporophores had less offensive aroma, improved texture and higher yield. The DNA sequences of these hybrids were found to be in the same clade as the P. citrinopileatus parent with a bootstrap value of 99 %. High bootstrap values indicate high genetic homology between hybrids and the P. citrinopileatus parent. The biological efficiencies of these hybrids P1xC9 (70.97 %) and P3xC8 (52.14 %) were also higher than the P. citrinopileatus parent (35.63 %). Interspecies hybrids obtained by this mating technique can lead to better strains of mushrooms for genetic improvement of the Pleurotus species.