The rpl5-rps14 mitochondrial region: a hot spot for DNA rearrangements in Solanum spp. somatic hybrids

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• 作者：N. Scotti ; L. Maréchal-Drouard and T. Cardi
• 关键词：Potato ; Somatic hybrids ; Mitochondrial DNA ; Homologous recombination ; rpl5–rps14 genes
• 刊名：Current Genetics
• 出版年：2004
• 出版时间：June 2004
• 年：2004
• 卷：45
• 期：6
• 页码：378-382
• 全文大小：332 KB
• 刊物类别：Biomedical and Life Sciences
• 刊物主题：Life Sciences
  Microbial Genetics and Genomics
  Microbiology
  Biochemistry
  Cell Biology
  Plant Sciences
  Proteomics
  
• 出版者：Springer Berlin / Heidelberg
• ISSN：1432-0983

文摘

Southern analysis with rpl5 and rps14 mtDNA gene probes of Solanum tuberosum, S. commersonii and a sample of somatic hybrids detected polymorphisms between parents and the appearance of a novel restriction fragment in various hybrids. In one of them, detailed mtDNA analyses revealed various configurations of the rpl5–rps14 region present at different stoichiometries. Multiple inter-parental recombination events across homologous sequences were assumed to have caused these rearrangements. Sequence similarity searches detected one sequence putatively involved in the recombination upstream of the rpl5 gene. The presence of a second recombinogenic sequence was inferred. We propose two models to explain the mechanism responsible for obtaining the different rpl5–rps14 arrangements shown after somatic hybridization. Variability in the rpl5–rps14 region observed in both the parental species and their somatic hybrids suggests this region is a hot spot for mtDNA rearrangements in Solanum spp.