Differential Immune Responses and Protective Effects in Avirulent Mycobacterial Strains Vaccinated BALB/c Mice

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• 作者：Laicheng Liu ; Ruiling Fu ; Xuefeng Yuan ; Chunwei Shi ; Shuling Wang…
• 关键词：Avirulent mycobacterial strains ; Mycobacterium bovis BCG ; Tuberculosis ; Vaccine
• 刊名：Current Microbiology
• 出版年：2015
• 出版时间：July 2015
• 年：2015
• 卷：71
• 期：1
• 页码：129-135
• 全文大小：557 KB
• 参考文献：1.Alsaadi AI, Smith DW (1973) The fate of virulent and attenuated mycobacteria in guinea pigs infected by the respiratory route. Am Rev Respir Dis 107:1041-046PubMed
  2.Annacker O, Pimenta-Araujo R, Burlen-Defranoux O, Barbos TC, Cumano A, Bandeira A (2001) CD25+ CD4+ T cells regulate the expansion of peripheral CD4 T cells through the production of IL-10. J Immunol 166:3008-018PubMed View Article
  3.Anonymous (2013) Global tuberculosis report. World Health Organization, Geneva
  4.Brandt L, Feino CJ, Weinreich OA, Chilima B, Hirsch P, Appelberg R, Andersen P (2002) Failure of the Mycobacterium bovis BCG vaccine: some species of environmental mycobacteria block multiplication of BCG and induction of protective immunity to tuberculosis. Infect Immun 70:672-78PubMed Central PubMed View Article
  5.Cooper AM (2009) Cell-mediated immune responses in tuberculosis. Annu Rev Immunol 27:393-22PubMed Central PubMed View Article
  6.Elias D, Britton S, Aseffa A, Engers H, Akuffo H (2008) Poor immunogenicity of BCG in helminth infected population is associated with increased in vitro TGF-beta production. Vaccine 26:3897-902PubMed View Article
  7.Falkinham JO 3rd (2009) Surrounded by mycobacteria: nontuberculous mycobacteria in the human environment. J Appl Microbiol 107:356-67PubMed View Article
  8.Fine PE (1995) Variation in protection by BCG: implications of and for heterologous immunity. Lancet 346:1339-345PubMed View Article
  9.Gonzalo Asensio J, Maia C, Ferrer NL, Barilone N, Laval F, Soto CY, Winter N, Daffé M, Gicquel B, Martín C, Jackson M (2006) The virulence-associated two-component PhoP-PhoR system controls the biosynthesis of polyketide-derived lipids in Mycobacterium tuberculosis. J Biol Chem 281:1313-316PubMed View Article
  10.Harboe M, Mshana RN, Closs O, Kronvall G, Axelsen NH (1979) Cross-reactions between mycobacteria. II. Crossed immunoelectrophoretic analysis of soluble antigens of BCG and comparison with other mycobacteria. Scand J Immunol 9:115-24PubMed View Article
  11.Hattikudur S, Kamat RS (1985) Polymorphism of a mycobacterial antigen participating in cell-mediated immunity. J Med Microbiol 19:69-5PubMed View Article
  12.Ho YS, Adroub SA, Abadi M, Al Alwan B, Alkhateeb R, Gao G, Ragab A, Ali S, van Soolingen D, Bitter W, Pain A, Abdallah AM (2012) Complete genome sequence of Mycobacterium vaccae type strain ATCC 25954. J Bacteriol 194:6339-340PubMed Central PubMed View Article
  13.Hougardy JM, Place S, Hildebrand M, Drowart A, Debrie AS, Locht C, Mascart F (2007) Regulatory T cells depress immune responses to protective antigens in active tuberculosis. Am J Respir Crit Care Med 176:409-16PubMed View Article
  14.Howard CJ, Kwong LS, Villarreal-Ramos B, Sopp P, Hope JC (2002) Exposure to Mycobacterium avium primes the immune system of calves for vaccination with Mycobacterium bovis BCG. Clin Exp Immunol 130:190-95PubMed Central PubMed View Article
  16.Kamala T, Paramasivan CN, Herbert D, Venkatesan P, Prabhakar R (1996) Immune response & modulation of immune response induced in the guinea-pigs by Mycobacterium avium complex (MAC) & M. fortuitum complex isolates from different sources in the south Indian BCG trial area. Indian J Med Res 103:201-11PubMed
  15.Howard ST, Byrd TF (2000) The rapidly growing mycobacteria: saprophytes and parasites. Microb Infect 2:1845-853View Article
  17.Larson CL, Wicht WC (1964) Infection of mice with Mycobacterium tuberculosis, strain H37Ra. Am Rev Respir Dis 90:742-48PubMed
  18.Li L, Lao SH, Wu CY (2007) Increased frequency of CD4 (+) CD25 (high) Treg cells inhibit BCG-specific induction of IFN-gamma by CD4 (+) T cells from TB patients. Tuberculosis (Edinb) 87:526-34View Article
  19.Lozes E, Denis O, Drowart A, Jurion F, Palfliet K, Vanonckelen A, De Bruyn J, De Cock M, Van Vooren JP, Huygen K (1997) Cross-reactive immune responses against Mycobacterium bovis BCG in mice infected with non-tuberculous mycobacteria belonging to the MAIS-Group. Scand J Immunol 46:16-6PubMed View Article
  20.Luo Y (2001) The immunotherapeutic effect of Mycobacterium vaccae vaccine on initially treated pulmonary tuberculosis. Zhonghua Jie He He Hu Xi Za Zhi 24:43-7 (in Chinese)PubMed
  21.Martin C, Williams A, Hernandez-Pando R, Cardona PJ, Gormley E, Bordat Y, Soto CY, Clark SO, Hatch GJ, Aguilar D, Ausina V, Gicquel B (2006) The live Mycobacterium tuberculosis phoP mutant strain is more attenuated than BCG and confers protective immunity against tuberculosis in mice and guinea pigs. Vaccine 24:3408-419PubMed View Article
  22.Nakamura K, Kitani A, Fuss I, Pedersen A, Harada N, Nawata H, Strober W (2004) TGF-beta 1 plays an important role in the mechanism of CD4+ CD25+ regulatory T cell activity in both humans and mice. J Immunol 172:834-42PubMed View Article
  23.Orme IM, Collins FM (1983) Infection with Mycobacterium kansasii and efficacy of vaccination against tuberculosis. Immunology 50:581-86PubMed
• 作者单位：Laicheng Liu (1)
  Ruiling Fu (2)
  Xuefeng Yuan (2)
  Chunwei Shi (2)
  Shuling Wang (3)
  Xianyu Lu (4)
  Zhao Ma (2)
  Xiaoming Zhang (2)
  Weiyan Qin (2)
  Xionglin Fan (2)
  
  1. Department of Medical Laboratory, Children’s Hospital of Chongqing Medical University, Ministry of Education Key Laboratory of Child Development and Disorders, Chongqing, 400014, People’s Republic of China
  2. Department of Pathogen Biology, School of Preclinical Medicine, Tongji Medical College, Huazhong University of Science & Technology, Wuhan, 430030, People’s Republic of China
  3. The Third People’s Hospital of Chongqing City, Clinical Laboratory Center of Chongqing, Chongqing, 400014, People’s Republic of China
  4. Department of Clinical Immunology, Chongqing Medical University, Chongqing, 400016, People’s Republic of China
  
• 刊物类别：Biomedical and Life Sciences
• 刊物主题：Life Sciences
  Microbiology
  Biotechnology
  
• 出版者：Springer New York
• ISSN：1432-0991

文摘

Screening live mycobacterial vaccine candidates is the important strategy to develop new vaccines against adult tuberculosis (TB). In this study, the immunogenicity and protective efficacy of several avirulent mycobacterial strains including Mycobacterium smegmatis, M. vaccae, M. terrae, M. phlei, M. trivial, and M. tuberculosis H37Ra were compared with M. bovis BCG in BALB/c mice. Our results demonstrated that differential immune responses were induced in different mycobacterial species vaccinated mice. As BCG-vaccinated mice did, M. terrae immunization resulted in Th1-type responses in the lung, as well as splenocytes secreting IFN-γ against a highly conserved mycobacterial antigen Ag85A. M. smegmatis also induced the same splenocytes secreting IFN-γ as BCG and M. terrae did. In addition, M. terrae and M. smegmatis-immunized mice predominantly increased expression of IL-10 and TGF-β in the lung. Most importantly, mice vaccinated with H37Ra and M. vaccae could provide the same protection in the lung against virulent M. tuberculosis challenge as BCG. The result may have important implications in developing adult TB vaccine.