Apoptosis induced by 3,7-dinitrodibenzobromonium salts in K562 cells

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• 作者：Ruidong Miao (1)
  Xiaohui Xia (1)
  Dongling Yang (2)
  Minghua Lü (1)
  Qin Wang (1)
  
• 关键词：3 ; 7 ; dinitrodibenzobromonium salts (cBr) ; anti ; tumor activity ; apoptosis
• 刊名：Frontiers in Biology
• 出版年：2008
• 出版时间：September 2008
• 年：2008
• 卷：3
• 期：3
• 页码：275-278
• 全文大小：131KB
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• 作者单位：Ruidong Miao (1)
  Xiaohui Xia (1)
  Dongling Yang (2)
  Minghua Lü (1)
  Qin Wang (1)
  
  1. School of Life Sciences, Lanzhou University, Lanzhou, 730000, China
  2. Institute of Biophysics, Chinese Academic of Sciences, Beijing, 100101, China
  
• ISSN：1674-7992

文摘

Abstract We evaluated the inhibitory effect of 3,7-dinitrodibenzobromonium salts (cBr) on the proliferation of human chronic myelogenous leukemia K562 cell by trypan blue exclusion test and MTT colorimetric assay. The degree of DNA damage in K562 cells treated with cBr, was detected by isotopic tracer method (3H-TdR). The morphological changes of these K562 cells were examined by fluorescence and electron microscopy. Biochemical characteristics of K562 cells were detected by flow cytometry and 3H-thymidine incorporation assay. Findings indicated that cBr could significantly inhibit cell proliferation and result in DNA damage of K562 cells. cBr is a new type of immunostimulant and can induce cell apoptosis.