A one-step ligation system for rapid generation of armed, conditionally-replicating adenoviruses

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• 作者：Xing Li (1)
  Ruiting Xia (2)
  Jing Zhao (1)
  Dongyang Wang (1)
  Qinwen Mao (3)
  Haibin Xia (1)
  
• 关键词：Adenovirus construction ; Conditionally ; replicating adenovirus ; Gene therapy ; Transgene
• 刊名：Biotechnology Letters
• 出版年：2013
• 出版时间：August 2013
• 年：2013
• 卷：35
• 期：8
• 页码：1215-1221
• 全文大小：584KB
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• 作者单位：Xing Li (1)
  Ruiting Xia (2)
  Jing Zhao (1)
  Dongyang Wang (1)
  Qinwen Mao (3)
  Haibin Xia (1)
  
  1. Laboratory of Gene Therapy, Department of Biochemistry, College of Life Sciences, Shaanxi Normal University, 199 South Chang鈥檃n Road, Xi鈥檃n, 710062, People鈥檚 Republic of China
  2. College of Liberal Arts and Sciences, University of Illinois at Urbana-Champaign, Urbana, IL, 61801, USA
  3. Department of Pathology, Northwestern University Feinberg School of Medicine, Chicago, IL, 60611, USA
  

文摘

Conditionally-replicating adenoviruses (CRAd) are becoming a promising strategy for cancer therapy. However, the generation of CRAd is complicated and time-consuming, especially for armed CRAd Ad5-D24. A one-step ligation system is now reported for rapid construction of an armed CRAd Ad5-D24 carrying two foreign transgenes. The system has the following features: (1) the CRAd backbone pCRAd5-D24-BS-Lacza contains two unique restriction sites between the fiber and E4 for easy introduction of a transgene expression cassette; and (2) a bi-directional promoter in pshuttle-BS-Dual allows for the expression of two independent transgenes. As proof of principle, Ad5-D24 CRAd carrying eGFP and mCherry was generated and examined. This novel ligation system will be a versatile and efficient tool in cancer viral gene therapy.