Express method for detection of Amanita phalloides amanitine toxins
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- 作者：D. N. Vlaskin ; E. T. Gainullina ; O. V. Klyuster ; I. V. Rybal¡¯chenko ; S. B. Ryzhikov and V. F. Taranchenko
- 关键词：amanitine ; rapid method ; fluorescence ; ethidium bromide
- 刊名：Bulletin of Experimental Biology and Medicine
- 出版年：2006
- 出版时间：January 2006
- 年：2006
- 卷：141
- 期：1
- 页码：110-111
- 全文大小：251 KB
文摘
Spectral studies of the interaction between amanitine and ethidium bromide fluorophore showed the appearance of a new intensive fluorescence band after addition of amanitine to ethidium bromide solution, caused by the formation of a charge-transfer complex. The new fluorescence band is located in a shorter wave region of the spectrum compared to ethidium bromide fluorescence band. Based on the results, a rapid fluorescent method for detection of amanitines was developed.