Krüppel-like Factor 5 contributes to pulmonary artery smooth muscle proliferation and resistance to apoptosis in human pulmonary arterial hypertension

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• 作者：Audrey Courboulin (1)
  Véronique L Tremblay (1)
  Marjorie Barrier (1)
  Jolyane Meloche (1)
  Maria Helena Jacob (1)
  Mathilde Chapolard (1)
  Malik Bisserier (1)
  Roxane Paulin (1)
  Caroline Lambert (1)
  Steeve Provencher (1)
  Sébastien Bonnet (1)
  
• 关键词：Pulmonary arterial hypertension ; KLF5 ; STAT3 ; proliferation ; apoptosis.
• 刊名：Respiratory Research
• 出版年：2011
• 出版时间：December 2011
• 年：2011
• 卷：12
• 期：1
• 全文大小：672KB
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• 作者单位：Audrey Courboulin (1)
  Véronique L Tremblay (1)
  Marjorie Barrier (1)
  Jolyane Meloche (1)
  Maria Helena Jacob (1)
  Mathilde Chapolard (1)
  Malik Bisserier (1)
  Roxane Paulin (1)
  Caroline Lambert (1)
  Steeve Provencher (1)
  Sébastien Bonnet (1)
  
  1. Department of Medicine, Faculty of Medicine, Laval University, Quebec, QC, Canada
  

文摘

Background Pulmonary arterial hypertension (PAH) is a vascular remodeling disease characterized by enhanced proliferation of pulmonary artery smooth muscle cell (PASMC) and suppressed apoptosis. This phenotype has been associated with the upregulation of the oncoprotein survivin promoting mitochondrial membrane potential hyperpolarization (decreasing apoptosis) and the upregulation of growth factor and cytokines like PDGF, IL-6 and vasoactive agent like endothelin-1 (ET-1) promoting PASMC proliferation. Krüppel-like factor 5 (KLF5), is a zinc-finger-type transcription factor implicated in the regulation of cell differentiation, proliferation, migration and apoptosis. Recent studies have demonstrated the implication of KLF5 in tissue remodeling in cardiovascular diseases, such as atherosclerosis, restenosis, and cardiac hypertrophy. Nonetheless, the implication of KLF5 in pulmonary arterial hypertension (PAH) remains unknown. We hypothesized that KLF5 up-regulation in PAH triggers PASMC proliferation and resistance to apoptosis. Methods and results We showed that KFL5 is upregulated in both human lung biopsies and cultured human PASMC isolated from distal pulmonary arteries from PAH patients compared to controls. Using stimulation experiments, we demonstrated that PDGF, ET-1 and IL-6 trigger KLF-5 activation in control PASMC to a level similar to the one seen in PAH-PASMC. Inhibition of the STAT3 pathway abrogates KLF5 activation in PAH-PASMC. Once activated, KLF5 promotes cyclin B1 upregulation and promotes PASMC proliferation and triggers survivin expression hyperpolarizing mitochondria membrane potential decreasing PASMC ability to undergo apoptosis. Conclusion We demonstrated for the first time that KLF5 is activated in human PAH and implicated in the pro-proliferative and anti-apoptotic phenotype that characterize PAH-PASMC. We believe that our findings will open new avenues of investigation on the role of KLF5 in PAH and might lead to the identification of new therapeutic targets.