An immunochemical test for rapid screening of zearalenone and T-2 toxin
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- 作者：Evgenia Yu. Basova ; Irina Yu. Goryacheva ; Tatiana Yu. Rusanova ; Natalia A. Burmistrova ; Richard Dietrich ; Erwin M?rtlbauer ; Christ¡¯l Detavernier ; Carlos Van Peteghem and Sarah De Saeger
- 关键词：Non ; instrumental test ; Visual detection ; Fusarium toxin ; Mycotoxin ; Zearalenone ; T ; 2 toxin ; Feed
- 刊名：Analytical and Bioanalytical Chemistry
- 出版年：2010
- 出版时间：May, 2010
- 年：2010
- 卷：397
- 期：1
- 页码：55-62
- 全文大小：235.5 KB
文摘
An immunochemically based test for non-instrumental simultaneous detection of zearalenone (ZEA) and T-2 toxin (T2) in feed was developed. The method combines clean-up of sample extract, pre-concentration of analytes by immunoextraction and immunodetection through the enzymatic reaction of horseradish peroxidase (HRP). The test is housed inside a standard 1-mL solid-phase extraction column and consists of three layers: two test layers (one for ZEA and another for T2) with immobilised specific antibodies and one control layer with bound anti-HRP antibodies. Feed extract was passed through an additional column with clean-up layer, which was disconnected after extract application. Total assay time was about 15 min for six samples and detection time was 4 min after chromogenic substrate application. Under optimised conditions a cut-off level for ZEA and T2 of 100 ¦Ìg/kg was established. Different feed types were analysed for ZEA and T2 contamination by the proposed method and results were confirmed by LC-MS/MS.