Development of a colloidal gold-based lateral-flow immunoassay for the rapid simultaneous detection of zearalenone and deoxynivalenol
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- 作者：Anna Yu Kolosova ; Sarah De Saeger ; Liberty Sibanda ; Ron Verheijen and Carlos Van Peteghem
- 关键词：Lateral ; flow immunoassay ; Colloidal gold ; Deoxynivalenol ; Zearalenone ; Multianalyte
- 刊名：Analytical and Bioanalytical Chemistry
- 出版年：2007
- 出版时间：December, 2007
- 年：2007
- 卷：389
- 期：7-8
- 页码：2103-2107
- 全文大小：118.9 KB
- 刊物类别：Chemistry and Materials Science
- 刊物主题：Chemistry
Analytical Chemistry
Food Science
Inorganic Chemistry
Physical Chemistry
Monitoring, Environmental Analysis and Environmental Ecotoxicology - 出版者：Springer Berlin / Heidelberg
- ISSN：1618-2650
文摘
A multianalyte lateral-flow technique using colloidal gold-labeled monoclonal antibodies was developed for the rapid simultaneous detection of deoxynivalenol (DON) and zearalenone (ZEA). The results of this qualitative one-step test were interpreted visually. A very simple and fast sample preparation was used, and the assay procedure could be accomplished within 10 min. When applied to spiked wheat samples, the technique gave accurate and reproducible results. Cut-off levels of 1500 and 100 μg kg−1 for DON and ZEA, respectively, were observed. The described multianalyte format can be used as a reliable, rapid and cost-effective on-site screening technique for the simultaneous determination of mycotoxins in grain samples.