T11TS inhibits glioma angiogenesis by modulation of MMPs, TIMPs, with related integrin αv and TGF-β1 expressions

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• 作者：Manoj Kumar Singh (1)
  Debanjan Bhattacharya (1)
  Suhnrita Chaudhuri (1)
  Sagar Acharya (2)
  Pankaj Kumar (1)
  Pranjal Santra (1)
  Anjan Kumar Basu (3)
  Swapna Chaudhuri (1)
  
• 关键词：Glioma ; Anti ; angiogenesis ; T11 target structure (T11TS) ; MMPs ; TIMPs ; Integrin αv
• 刊名：Tumor Biology
• 出版年：2014
• 出版时间：March 2014
• 年：2014
• 卷：35
• 期：3
• 页码：2231-2246
• 全文大小：4,249 KB
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• 作者单位：Manoj Kumar Singh (1)
  Debanjan Bhattacharya (1)
  Suhnrita Chaudhuri (1)
  Sagar Acharya (2)
  Pankaj Kumar (1)
  Pranjal Santra (1)
  Anjan Kumar Basu (3)
  Swapna Chaudhuri (1)
  
  1. Department of Laboratory Medicine, School of Tropical Medicine, 108, C.R. Avenue, Kolkata, 700073, West Bengal, India
  2. Department of Zoology, Vidyasagar University, West Midnapore, West Bengal, India
  3. Department of Biochemistry and Medical Biotechnology, School of Tropical Medicine, 108, C.R. Avenue, Kolkata, 700073, West Bengal, India
  
• ISSN：1423-0380

文摘

During glioma development, angiogenesis plays a crucial role in growth and vascularization of primary brain tumors. T11 target structure (T11TS), a bioactive molecule, has been documented as an anti-neoplastic agent in glioma-induced rats and also in human glioma in vitro. This novel molecule induces apoptosis of tumor cells by way of immune potentiation and impairs the glioma cell cycle, but its role in glioma angiogenesis has not been worked out in detail. Matrix metalloproteinases (MMPs) are enzymes promoting tumor angiogenesis by enzymatically remodeling the extracellular matrix and altering surface protein expression such as integrin αv and the matrix-bound proteins like TGF-β1. The present study was formulated to assess the efficacy of T11TS in the modulations of MMP-2 and ? and their endogenous inhibitors (TIMP-1 and TIMP-2) as well as modulations of integrin αv and TGF-β1 in glioma-induced rats and also on the phenotypic markers of endothelial cells (CD31 and CD34). The parameters used were zymography, western blot, and flow cytometric analyses. It was observed that T11TS administration significantly downregulates the expression of matrix metalloproteinase-2 and ? along with its ligand integrin αv and upregulates TIMP-1 and TIMP-2. In situ immunofluorescence and FACS results revealed that T11TS administration decreased the expression of the phenotypic markers (CD31/PECAM1, CD34), inhibiting the cell grip and also downregulating TGF-β1 expression (ELISA) from microglia cells in the glioma microenvironment. These results suggest that T11TS suppresses the expression of positive angiogenic growth factors and potentiates the expression of negative regulators in glioma-associated endothelial cells (ECs), resulting in an anti-angiogenic effect on glioma-induced angiogenesis.