Ectopic overexpression of a mungbean vacuolar Na+/H+ antiporter gene (VrNHX1) leads to increased salinity stress tolerance in transgenic Vi
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- 作者：Sagarika Mishra ; Ratikanta Behura ; Jay Prakash Awasthi ; Mohitosh Dey…
- 关键词：Salt tolerance ; Vacuolar sodium/proton antiporter ; VrNHX1 ; Transgenic cowpea
- 刊名：Molecular Breeding
- 出版年：2014
- 出版时间：October 2014
- 年：2014
- 卷：34
- 期：3
- 页码：1345-1359
- 全文大小：3,167 KB
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Ratikanta Behura (1)
Jay Prakash Awasthi (1) (2)
Mohitosh Dey (1)
Debeeprasad Sahoo (1)
Sudipta Shekhar Das Bhowmik (1) (3)
Sanjib Kumar Panda (2)
Lingaraj Sahoo (1)
1. Department of Biotechnology, Indian Institute of Technology Guwahati, Guwahati, 781039, India
2. Department of Life Sciences and Bioinformatics, Assam University, Silchar, 788011, India
3. Center for Tropical Crops and Biocommodities, Queensland University of Technology, Gardens Point Campus, Brisbane, QLD, 4059, Australia - ISSN：1572-9788
文摘
Salinity is a major threat to sustainable agriculture worldwide. Plant NHX exchangers play an important role in conferring salt tolerance under salinity stress. In this study, a vacuolar Na+/H+ antiporter gene VrNHX1 (Genbank Accession No. JN656211.1) from mungbean (Vigna radiata) was introduced into cowpea (Vigna unguiculata) by the Agrobacterium tumefaciens-mediated transformation method. Polymerase chain reaction and Southern blot hybridization confirmed the stable integration of VrNHX1 into the cowpea genome. Comparative expression analysis by semi-quantitative RT-PCR revealed higher expression of VrNHX1 in transgenic cowpea plants than wild-type. Under salt stress conditions, T2 transgenic 35S:VrNHX1 cowpea lines exhibited higher tolerance to 200?mM NaCl treatment than wild-type. Furthermore, T2 transgenic 35S:VrNHX1 lines maintained a higher K+/Na+ ratio in the aerial parts under salt stress and accumulated higher [Na+] in roots than wild-type. Physiological analysis revealed lower levels of lipid peroxidation, hydrogen peroxide and oxygen radical production but higher levels of relative water content and proline, ascorbate and chlorophyll contents in T2 transgenic 35S:VrNHX1 lines.