Metabolic and Co-Metabolic Transformation of Diclofenac by Enterobacter hormaechei D15 Isolated from Activated Sludge

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• 作者：Salima Aissaoui ; Houria Ouled-Haddar ; Mohamed Sifour…
• 关键词：Biodegradation ; Diclofenac ; Enterobacter hormaechei ; 1 ; (2 ; 6 dichlophenyl) ; 1.3 ; dihydro ; 2H ; indol.2.one ; Co ; metabolism ; GCMS
• 刊名：Current Microbiology
• 出版年：2017
• 出版时间：March 2017
• 年：2017
• 卷：74
• 期：3
• 页码：381-388
• 全文大小：
• 刊物类别：Biomedical and Life Sciences
• 刊物主题：Microbiology; Biotechnology;
• 出版者：Springer US
• ISSN：1432-0991
• 卷排序：74

文摘

The presence of non-steroidal anti-inflammatory drugs, such as diclofenac (DCF), in the environment, is an emerging problem due to their harmful effects on non-target organisms, even at low concentrations. We studied the biodegradation of DCF by the strain D15 of Enterobacter hormaechei. The strain was isolated from an activated sludge, and identified as E. hormaechei based on its physiological characteristics and its 16 S RNA sequence. Using HPTLC and GC-MS methods, we demonstrated that this strain metabolized DCF at an elimination rate of 52.8%. In the presence of an external carbon source (glucose), the elimination rate increased to approximately 82%. GC-MS analysis detected and identified one metabolite as 1-(2,6-dichlorophenyl)-1,3-dihydro-2H-indol-2-one; it was produced as a consequence of dehydration and lactam formation reactions.