Absolute qPCR for Measuring Telomere Length in Bone Marrow Samples of Plasma Cell Disorders

详细信息    查看全文

• 作者：Julieta Panero ; Nathan J. O’Callaghan ; Michael Fenech…
• 关键词：Telomere length ; Absolute quantification ; Bone marrow samples ; Multiple myeloma ; MGUS
• 刊名：Molecular Biotechnology
• 出版年：2015
• 出版时间：February 2015
• 年：2015
• 卷：57
• 期：2
• 页码：155-159
• 全文大小：263 KB
• 参考文献：1. Counter, C. M., Avilion, A. A., LeFeuvre, C. E., et al. (1992). Telomere shortening associated with chromosome instability is arrested in immortal cells which express telomerase activity. / EMBO Journal, / 11, 1921-929.
  2. Wu, X., Amos, C. I., Zhu, Y., et al. (2003). Telomere dysfunction: A potential cancer predisposition factor. / Journal of the National Cancer Institute, / 95, 1211-218. CrossRef
  3. Hahn, W. C. (2003). Role of telomeres and telomerase in the pathogenesis of human cancer. / Journal of Clinical Oncology, / 21, 2034-043. CrossRef
  4. Stewart, S. A., & Weinberg, R. A. (2006). Telomeres: Cancer to human aging. / Annual Review of Cell and Developmental Biology, / 22, 531-57. CrossRef
  5. Allshire, R. C., Dempster, M., & Hastie, N. D. (1989). Human telomeres contain at least three types of G-rich repeat distributed non-randomly. / Nucleic Acids Research, / 17, 4611-627. CrossRef
  6. Cawthon, R. M. (2002). Telomere measurement by quantitative PCR. / Nucleic Acids Research, / 30, e47. CrossRef
  7. O’Callaghan, N., Dhillon, V., Thomas, P., & Fenech, M. (2008). A quantitative real-time PCR method for absolute telomere length. / BioTechniques, / 44, 807-09. CrossRef
  8. O’Callaghan, N., & Fenech, M. (2011). A quantitative PCR method for measuring absolute telomere length. / Biological Procedures Online, / 13, 3. CrossRef
  9. Folini, M., Venturini, L., Cimino-Reale, G., & Zaffaroni, N. (2011). Telomeres as targets for anticancer therapies. / Expert Opinion on Therapeutic Targets, / 15, 579-93.
  10. Greipp, P. R., San Miguel, J., Durie, B. G., et al. (2005). International staging system for multiple myeloma. / Journal of Clinical Oncology, / 23, 3412-420. CrossRef
  11. Cottliar, A., Pedrazzini, E., Corrado, C., Engelberger, M. I., Narbaitz, M., & Slavutsky, I. (2003). Telomere shortening in patients with plasma cell disorders. / European Journal of Haematology, / 71, 334-40. CrossRef
  12. Baird, D. M. (2005). New developments in telomere length analysis. / Experimental Gerontology, / 40, 363-68. CrossRef
  13. Wu, K. D., Orme, L. M., Shaughnessy, J, Jr, Jacobson, J., Barlogie, B., & Moore, M. A. (2003). Telomerase and telomere length in multiple myeloma: Correlations with disease heterogeneity, cytogenetic status, and overall survival. / Blood, / 101, 4982-989. CrossRef
  14. Panero, J., Arbelbide, J., Fantl, D., Rivello, H. G., Kohan, D., & Slavutsky, I. (2010). Altered mRNA expression of telomere-associated genes in monoclonal gammopathy of undetermined significance and multiple myeloma. / Molecular Medicine, / 16, 471-78. CrossRef
  15. Jones, C. H., Pepper, C., & Baird, D. M. (2012). Telomere dysfunction and its role in haematological cancer. / British Journal of Haematology, / 156, 573-87. CrossRef
  
• 刊物主题：Biotechnology; Biochemistry, general; Cell Biology; Protein Science; Biological Techniques; Human Genetics;
• 出版者：Springer US
• ISSN：1559-0305

文摘

Telomere length (TL) is currently used as an emerging biomarker in understanding the development/progression of hematological malignancies. The absolute quantitative PCR (qPCR) methodology has allowed the study of TL from a variety of mammalian tissues, but it has not been tested for bone marrow (BM) samples. In this study, we have examined the relationship between TL data generated by absolute qPCR versus those obtained by terminal restriction fragments (TRF) in 102?BM samples from patients with plasma cell disorders. A significant linear correlation between both methodologies was observed (p?r 2?=?0.70). Results were also analyzed in relation to clinical characteristics and significant associations between telomere shortening and parameters of adverse prognosis were observed. Furthermore, another set of 47?BM samples from patients with low quantity of DNA for TRF assay were suitably analyzed by qPCR, indicating the usefulness of the absolute qPCR methodology for the inclusion of patients with scarce material to the study. Taken together, these findings are of interest considering the importance of telomere dysfunction in the pathogenesis of cancer and give a new alternative to measure TL in hematologic disorders with substantial time and cost savings.