Repeated randomized selection of genotypes for reliable estimates of population differentiation in data containing siblings

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• 作者：Patrick P. Lenhardt ; Kathrin Theissinger
• 关键词：Modelling ; genetic population differentiation ; Larvae sampling ; Full ; siblings ; Microsatellites
• 刊名：European Journal of Wildlife Research
• 出版年：2017
• 出版时间：February 2017
• 年：2017
• 卷：63
• 期：1
• 全文大小：
• 刊物类别：Biomedical and Life Sciences
• 刊物主题：Zoology; Ecology; Fish & Wildlife Biology & Management;
• 出版者：Springer Berlin Heidelberg
• ISSN：1439-0574
• 卷排序：63

文摘

In population genetic studies, a proper sampling design is crucial for reliable population differentiation estimates. For genetic studies on amphibians, fish or insects, larvae are often sampled instead of adults due to their higher accessibility and abundance. However, population genetic parameters derived from larval (sibling) sampling can be biased if adults are represented unevenly in the larval population. The removal of full-siblings from data may improve the quality of the results but entails in a low number of individuals per site, especially in small populations. Using simulated data of ten microsatellite loci for ten populations, we estimated pairwise F_ST and R_ST values as well as F_IS and R_IS values for the F0 generation (parental) and the F1 generation (descendant). We applied a repeated randomized selection of genotypes (RRSG) to investigate the impact of removing full-siblings from the data. We also investigated the impact of reduced sample size on the results generated by RRSG as well as the advantages of RRSG over a single estimate of genetic parameters after removal of full-siblings. The RRSG approach produced pairwise F_ST values that deviated on average only by 0.0050 (N = 45) from their respective estimates for F0. Estimates for H_E deviated less the 5% over all data sets. We henceforth suggest applying the described method to interpretations of genetic differentiations in studies with (i) small effective population sizes and (ii) data containing siblings where (iii) offspring can be assigned to at least one parent due to reproduction practice.