Physiological performance, secondary metabolite and expression profiling of genes associated with drought tolerance in Withania somnifera

详细信息    查看全文

• 作者：Sanchita ; Ruchi Singh ; Anand Mishra ; Sunita S. Dhawan ; Pramod A. Shirke…
• 关键词：Withanolides ; Water stress ; Withania somnifera ; Gene expression ; Photosynthesis rate
• 刊名：Protoplasma
• 出版年：2015
• 出版时间：November 2015
• 年：2015
• 卷：252
• 期：6
• 页码：1439-1450
• 全文大小：1,556 KB
• 参考文献：Agarwal PK, Shukla PS, Gupta K, Jha B (2013) Bioengineering for salinity tolerance in plants: state of the art. Mol Biotechnol 54(1):102-23CrossRef PubMed
  Alscher RG, Erturk N, Heath LS (2002) Role of superoxide dismutases (SODs) in controlling oxidative stress in plants. J Exp Bot 53(372):1331-341CrossRef PubMed
  Alvarez S, Zhu M, Chen S (2009) Proteomics of Arabidopsis redox proteins in response to methyl jasmonate. J Proteomics 73(1):30-0CrossRef PubMed
  Asada K (1999) The water-water cycle in chloroplasts: scavenging of active oxygens and dissipation of excess photons. Annu Rev Plant Physiol Plant Mol Biol 50:601-39CrossRef PubMed
  Bates LS, Waldren RP, Teare ID (1973) Rapid determination of free proline for water-stress studies. Plant Soil 39(1):205-07CrossRef
  Bhaskara GB, Nguyen TT, Verslues PE (2012) Unique drought resistance functions of the highly ABA-induced clade A protein phosphatase 2Cs. Plant Physiol 160(1):379-95PubMedCentral CrossRef PubMed
  Boyer JS, James RA, Munns R, Condon T, Passioura JB (2008) Osmotic adjustment leads to anomalously low estimates of relative water content in wheat and barley. Funct Plant Biol 35(11):1172-182CrossRef
  Burke JJ, Hatfield JL, Klein RR, Mullet JE (1985) Accumulation of heat shock proteins in field-grown cotton. Plant Physiol 78(2):394-98PubMedCentral CrossRef PubMed
  Ceulemans H, Bollen M (2004) Functional diversity of protein phosphatase-1, a cellular economizer and reset button. Physiol Rev 84(1):1-9CrossRef PubMed
  Chalker-Scott L (1999) Environmental significance of anthocyanins in plant stress responses. J Photochem Photobiol 70(1):1-CrossRef
  Chaurasiya ND, Uniyal GC, Lal P, Misra L, Sangwan NS, Tuli R, Sangwan RS (2008) Analysis of withanolides in root and leaf of Withania somnifera by HPLC with photodiode array and evaporative light scattering detection. Phytochem Anal 19(2):148-54CrossRef PubMed
  Chen H, Lai Z, Shi J, Xiao Y, Chen Z, Xu X (2010) Roles of Arabidopsis WRKY18, WRKY40 and WRKY60 transcription factors in plant responses to abscisic acid and abiotic stress. BMC Plant Biol 10:281PubMedCentral CrossRef PubMed
  Chen JH, Jiang HW, Hsieh EJ, Chen HY, Chien CT, Hsieh HL, Lin TP (2012) Drought and salt stress tolerance of an Arabidopsis glutathione S-transferase U17 knockout mutant are attributed to the combined effect of glutathione and abscisic acid. Plant Physiol 158(1):340-51PubMedCentral CrossRef PubMed
  Cruz de Carvalho MH (2008) Drought stress and reactive oxygen species: production, scavenging and signaling. Plant Signal Behav 3(3):156-65PubMedCentral CrossRef PubMed
  Dall'Osto L, Cazzaniga S, Havaux M, Bassi R (2010) Enhanced photoprotection by protein-bound vs free xanthophyll pools: a comparative analysis of chlorophyll b and xanthophyll biosynthesis mutants. Mol Plant 3(3):576-93CrossRef PubMed
  Demmig-Adams B, Adams WW 3rd (2006) Photoprotection in an ecological context: the remarkable complexity of thermal energy dissipation. New Phytol 172(1):11-1CrossRef PubMed
  Denga G, Lianga J, Xua D, Longa H, Pana ZH, Yua M (2013) The relationship between proline content, the expression level of P5CS (Δ1-pyrroline-5-carboxylate synthetase), and drought tolerance in Tibetan hulless barley (Hordeum vulgare var. nudum). Russ J Plant Physl 60(5):693-00CrossRef
  Dinakar C, Djilianov D, Bartels D (2012) Photosynthesis in desiccation tolerant plants: energy metabolism and antioxidative stress defense. Plant Sci 182:29-1CrossRef PubMed
  Faize M, Burgos L, Faize L, Piqueras A, Nicolas E, Barba-Espin G, Clemente-Moreno MJ, Alcobendas R, Artlip T, Hernandez JA (2011) Involvement of cytosolic ascorbate peroxidase and Cu/Zn-superoxide dismutase for improved tolerance against drought stress. J Exp Bot 62(8):2599-613CrossRef PubMed
  Fan SL, Yuan ZH, Feng LJ, Wang XH, Ding XM, Zhen HL (2011) Effects of drought stress on physiological and biochemical parameters of Dahlia pinnata. Ying Yong Sheng Tai Xue Bao 22(3):651-57PubMed
  Gill SS, Tuteja N (2010) Reactive oxygen species and antioxidant machinery in abiotic stress tolerance in crop plants. Plant Physiol Bioch 48(12):909-30
  Heath RL, Packer L (1968) Photoperoxidation in isolated chloroplasts. I. Kinetics and stoichiometry of fatty acid peroxidation. Arch Biochem Biophys 125(1):189-98CrossRef PubMed
  Hogewoning SW, Wientjes E, Douwstra P, Trouwborst G, van Ieperen W, Croce R, Harbinson J (2012) Photosynthetic quantum yield dynamics: from photosystems to leaves. Plant Cell 24(5):1921-935PubMedCentral CrossRef PubMed
  Huang W, Ma X, Wang Q, Gao Y, Xue Y, Niu X, Yu G, Liu Y (2008) Significant improvement of stress tolerance in tobacco plants by overexpressing a stress-responsive aldehyde dehydrogenase gene from maize (Zea mays). Plant Mol Biol 68(4-):451-63CrossRef PubMed
  Johnson SM, Doherty SJ, Croy RRD (2003) Biphasic superoxide generation in potato tubers. A self-amplifying response to stress. Plant Physiol
• 作者单位：Sanchita (1)
  Ruchi Singh (2)
  Anand Mishra (1)
  Sunita S. Dhawan (1)
  Pramod A. Shirke (2)
  Madan M. Gupta (3)
  Ashok Sharma (1)
  
  1. Biotechnology Division, CSIR–Central Institute of Medicinal and Aromatic Plants, Lucknow, India
  2. Plant Physiology Division, CSIR–National Botanical Research Institute, Lucknow, India
  3. Chemical Sciences Division, CSIR–Central Institute of Medicinal and Aromatic Plants, Lucknow, India
  
• 刊物类别：Biomedical and Life Sciences
• 刊物主题：Life Sciences
  Cell Biology
  Plant Sciences
  Zoology
  
• 出版者：Springer Wien
• ISSN：1615-6102

文摘

Physiological, biochemical, and gene expression responses under drought stress were studied in Withania somnifera. Photosynthesis rate, stomatal conductance, transpiration rate, relative water content, chlorophyll content, and quantum yield of photosystems I and II (PSI and PSII) decreased in response to drought stress. Comparative expression of genes involved in osmoregulation, detoxification, signal transduction, metabolism, and transcription factor was analyzed through quantitative RT–PCR. The genes encoding 1-pyrroline-5-carboxylate synthetase (P5CS), glutathione S-transferase (GST), superoxide dismutase (SOD), serine threonine-protein kinase (STK), serine threonine protein phosphatase (PSP), aldehyde dehydrogenase (AD), leucoanthocyanidin dioxygenase/anthocyanin synthase (LD/AS), HSP, MYB, and WRKY have shown upregulation in response to drought stress condition in leaf tissues. Enhanced detoxification and osmoregulation along with increased withanolides production were also observed under drought stress. The results of this study will be helpful in developing stress-tolerant and high secondary metabolite yielding genotypes. Keywords Withanolides Water stress Withania somnifera Gene expression Photosynthesis rate