Morphological, physiological and biochemical adaptations of Eucalyptus citriodora seedlings under NaCl stress in hydroponic conditions

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• 作者：Anusha Pulavarty ; Sanchita Kukde ; Vilas M. Shinde…
• 关键词：Salt tolerance ; Growth response ; Biomass accretion ; Sodium accumulation ; Osmolyte accumulation
• 刊名：Acta Physiologiae Plantarum
• 出版年：2016
• 出版时间：January 2016
• 年：2016
• 卷：38
• 期：1
• 全文大小：1,718 KB
• 参考文献：Agres (1994) Agres Statistical Software Version 3.01. Pascal International Software Solutions, USA
  Ahmad R, Kim MD, Back KH, Kim HS, Lee HS, Kwon SY, Murata N, Chung WI, Kwak SS (2008) Stress-induced expression of choline oxidase in potato plant chloroplasts confers enhanced tolerance to oxidative, salt, and drought stresses. Plant Cell Rep 27:687–698CrossRef PubMed
  Ahmed HE, Youssef EA, Kord MA, Qaid EA (2013) Trehalose accumulation in wheat plant promotes sucrose and starch biosynthesis. Jor J Biol Sci 6:143–150
  Akram MS, Ali Q, Athar HR, Bhatti AS (2006) Ion uptake and distribution in Panicum antidotale retz. under salt stress. Pak J Bot 38:1661–1669
  Ashraf M, Harris PJC (2004) Potential biochemical indicators of salinity tolerance in plants. Plant Sci 166:3–16CrossRef
  Ashraf M, Nazir N, McNeilly T (2001) Comparative salt tolerance of amphidiploid and diploid Brassica species. Plant Sci 160:683–689CrossRef PubMed
  Bandeh-hagh A, Toorchi M, Mohammadi A, Chaparzadeh N, Salekdeh GH, Kazemnia H (2008) Growth and osmotic adjustment of canola genotypes in response to salinity. J Food Agr Environ 6:201–208
  Bashiti TE, Hamamci H, Oktem HA, Yucel M (2005) Biochemical analysis of trehalose and its metabolizing enzymes in wheat under abiotic stress conditions. Plant Sci 169:47–54CrossRef
  Bates LS, Waldren RP, Teare LD (1973) Rapid determination of free proline for water stress studies. Plant Soi 39:205–207CrossRef
  Carillo P, Parisi D, Woodrow P, Pontecorvo G, Massaro G, Annunziata MG, Fuggi A, Sulpice R (2011) Salt induced accumulation of Glycine betaine is inhibited by high light in Durum wheat. Func Plan Biol 38:139–150CrossRef
  Cha-um S, Somsueb S, Samphumphuang T, Kirdmanee C (2013) Salt tolerant screening in eucalypt genotypes (Eucalyptus spp.) using photosynthetic abilities, proline accumulation, and growth characteristics as effective indices. In Vitro Cell Dev Biol 49:611–619CrossRef
  Chaves MM, Flexas J, Pinheiro C (2009) Photosynthesis under drought and salt stress: regulation mechanisms from whole plant to cell. Ann Bot 103:551–560PubMedCentral CrossRef PubMed
  Chen TH, Murata N (2002) Enhancement of tolerance of abiotic stress by metabolic engineering of betaines and other compatible solutes. Curr Opin Plant Biol 5:250–257CrossRef PubMed
  Chookhampaeng S (2011) The effect of salt stress on growth, chlorophyll content, proline content and antioxidative enzymes of pepper (Capsicum annuum L.) seedling. European J of Scien Resear 49:103–109
  Dutta BK, Karmakar S, Naglot A, Aich JC, Begam M (2007) Anticandidial activity of some essential oils of a mega biodiversity hotspot in India. Mycoses 50:121–124CrossRef PubMed
  Feikema PM, Sasse JM, Bandara GD (2012) Chloride content and biomass partitioning in Eucalyptus hybrids grown on saline sites. New Forest 43:89–107CrossRef
  Gadallah MAA (1999) Effects of proline and glycinebetaine on Vicia faba responses to salt stress. Biol Plant 42:249–257CrossRef
  Gebauer J, Siddigb KE, Salihc AA, Ebert G (2004) Tamarindus indica L. seedlings are moderately salt tolerant when exposed to NaCl-induced salinity. Scientia Horticult 103:1–8CrossRef
  Gebre GM, Tschaplinski TJ, Tuskan GA, Todd DE (1998) Clonal and seasonal differences in leaf osmotic potential and organic solutes of five hybrid poplar clones grown under field conditions. Tree Physiol 18:645–652CrossRef PubMed
  Ghoulam C, Foursy A, Khalid F (2002) Effects of salt stress on growth, inorganic ions and proline accumulation in relation to osmotic adjustment in five sugar beet cultivars. Environ Exp Bot 47:39–50CrossRef
  Gucci R, Lombardini L, Tattini M (1997) Analysis of leaf water relations of two olive (Olea europea) cultivars differing in tolerance to salinity. Tree Physiol 17:13–21CrossRef PubMed
  Heidari A, Toorchi M, Bandehagh A, Shakiba MR (2011) Effect of NaCl stress on growth, water relations, organic and inorganic osmolytes accumulation in Sunflower (Helianthus annuus L.) Lines. Univ J Environ Res Technol 1:351–362
  Javid MG, Sorooshzadeh A, Moradi F, Sanavy SAMM, Allahdadi I (2011) The role of phytohormone in alleviating salt stress in crop plants. Aust J Crop Sci 5:726–734
  Juhany LIE, Aref IM, Ahmed AIM (2008) Response of Eucalyptus camaldulensis, Eucalyptus microtheca and Eucalyptus intertexta Seedlings to Irrigation with Saline Water. World J Agr Sci 4:825–834
  Kong Y, Zhou G, Wang Y (2001) Physiological characteristics and alternative respiratory pathway under stress in two wheat cultivars differing in salt tolerance. Russ J Plant Physiol 48:595–600CrossRef
  Marcar NE, Termaat A (1990) Effect of root zone solutes on Eucalyptus camaldulensis and Eucalyptus bicostata seedlings: responses to Na+, Mg2+ and Cl−. Plant Soil 125:245–254CrossRef
  Marschner H (1995) Mineral Nutrition of Higher Plants. Academic Press, London
  Matsunaga E, Nanto K, Oishi M, Ebinuma H, Morishita Y, Sakurai N, Suzuki H, Shibata D, Shimada T (2012) Agrobacterium-mediated transformation of Eucalyptus globulus using explants with shoot apex with introduction of bacterial choline oxidase gene to enhance salt tolerance. Plant Cell Rep 31:225–235CrossRef PubMed
  Merchant A, Callister A, Arndt S, Tausz M, Adams M (2007) Contrasting physiological responses of six eucalyptus species to water deficit. Oxford J Ann Bot 100:1507–1515CrossRef
  Morabito D, Mills D, Prat D, Dizengremel P (1994) Response of clones of Eucalyptus microtheca to NaCl in vitro. Tree Physiol 14:201–210CrossRef PubMed
  Nasim M, Qureshi RH, Aziz T, Saqib M, Nawaz S, Sahi ST, Pervaiz S (2008) Growth and ionic composition of salt-stressed Eucalyptus camaldulensis and Eucalyptus tereticornis. Pak J Bot 40:799–805
  Nguyen A, Lamant A (1988) Pinitol and myo-inositol accumulation in water-stressed seedlings of maritime pine. Phytochem 27:3423–3427CrossRef
  Popp M, Lied W, Bierbaum U, Gross M, Große-Schulte T, Hams S, Oldenettel J, Schüler S, Wiese J (1997) Cyclitols—stable osmotica in trees. In: Rennenberg H, Escrich S, Ziegler H (eds) Trees—contributions to modern tree physiology. Backhuys Publishers, Leiden, pp 257–270
  Sarwar MKS, Ullah I, Rahman MU, Asraf MY, Zafar Y (2006) Glycine betaine accumulation and its relation to yield and yield components in cotton genotypes grown under water deficit condition. Pak J Bot 38:1449–1456
  Shannon MC, Grieve CM (1999) Tolerance of vegetable crops to salinity. Scientia Hort 78:5–38CrossRef
  Siddique MRB, Hamid A, Islam MS (2000) Drought stress effects on water relations of wheat. Bot Bull Acad Sin 41:35–39
  Silva ALLD, Oliveira YD, Dibax R, Costa JDL, Scheidt GN, Machado MP, Guerra EP, Brondani GE, Alves SAO (2012) Hydroponics growth of Eucalyptus saligna Sm. on salt-stress mediated by sodium chloride. J Bact Biotech 3:213–218
  Silveira JAG, Viegas RA, Rocha IMA, Moreira ACDM, Moreira RA, Oliveira JTA (2003) Proline accumulation and glutamine synthetase activity are increased by salt-induced proteolysis in cashew leaves. J Plant Physiol 160:115–123CrossRef PubMed
  Singh A, Toky OP, Singh B, Dhillon GPS (2011) Influence of salinity on biomass and plant water relations of Eucalyptus tereticornis and E. camaldulensis. Indian J of Ecology 38:180–183
  Tahir MA, Aziz RT, Ashraf M, Kanwal S, Maqsood MA (2006) Beneficial effects of silicon in wheat (Triticum aestivum L.) under salinity stress. Pak J Bot 38:1715–1722
  Turan MA, Elkarim AHA, Taban A, Taban S (2010) Effect of salt stress on growth and ion distribution and accumulation in shoot and root of maize plant. Afr J Agric Res 5:584–588
  Witham FH, Blaydes BF, Devlin RM (1971) Experiments in plant physiology. Van Nostrand Reinhold, New York, pp 167–200
  Woodward AJ, Bennett IJ (2005) The effect of salt stress and abscisic acid on proline production, chlorophyll content and growth of in vitro propagated shoots of Eucalyptus camaldulensis. Plant Cell Tiss Org Cult 82:189–200CrossRef
  Yang CW, Zhang ML, Liu J, Shi DC, Wang DL (2009) Effects of buffer capacity on growth, photosynthesis, and solute accumulation of a glycophyte (wheat) and a halophyte (Chlorisvirgata). Photosynthetica 47:55–60CrossRef
  Zhu JK (2003) Regulation of ion homeostasis under salt stress. Curr Opin Plant Biol 6:441–445CrossRef PubMed
  Zohar Y, Stefano JD, Bartle J (2010) Strategy for screening Eucalypts for saline lands. Agroforest Syst 78:127–137CrossRef
  
• 作者单位：Anusha Pulavarty (1)
  Sanchita Kukde (1)
  Vilas M. Shinde (2)
  Bijaya Ketan Sarangi (1)
  
  1. Environmental Biotechnology Division, CSIR-National Environmental Engineering Research Institute, Nehru Marg, Nagpur, 440020, Maharashtra, India
  2. Analytical Instrumentation Division, CSIR-National Environmental Engineering Research Institute, Nehru Marg, Nagpur, 440020, Maharashtra, India
  
• 刊物主题：Plant Physiology; Plant Genetics & Genomics; Plant Biochemistry; Plant Pathology; Plant Anatomy/Development; Agriculture;
• 出版者：Springer Berlin Heidelberg
• ISSN：1861-1664

文摘

Salinity stress could be managed by the growth of economically important tree species. Adaptations of Eucalyptus citriodora to NaCl stress was investigated under different concentrations of NaCl (15–75 mM). After 2 and 6 months of treatments, data were recorded for growth performance (shoot and root length, leaf number and area), physiological attributes [chlorophyll _a (Chl _a ), chlorophyll _b (Chl _b ), total chlorophyll (TC), carotenoids, relative water content (RWC)] and osmolyte accumulation [proline, glycine betaine (GB) and trehalose] parameters. After 2 months, changes in morphological parameters of treated plants were negligible (1–1.8-folds) in comparison to control, which is correlated with 2–8-, 1.12–7.7- and 1.73–3.94-folds increase in GB, proline and trehalose contents, respectively. At 6 months, though there was plant mortality, 70–80 % decrease in TC contents and reduction in osmolyte contents but 20 % increase in RWC of stressed plants were monitored. Survival at higher salt concentrations with accumulation of sodium shows significant salt tolerance in this species. Keywords Salt tolerance Growth response Biomass accretion Sodium accumulation Osmolyte accumulation