Regeneration of plants from leaflet explants of tissue culture raised safed siris (Albizia procera)
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- 作者：Sandeep Kumar ; A.K. Sarkar and C. Kunhikannan
- 关键词：Albizia procera ; BA ; leaf explant ; Phytagel ; shoot bud regeneration ; root initiation
- 刊名：Plant Cell, Tissue and Organ Culture
- 出版年：1998
- 出版时间：1998
- 年：1998
- 卷：54
- 期：3
- 页码：137-143
- 全文大小：167 KB
文摘
Shoot bud regeneration was obtained from isolated leaflets of Albizia procera cultured on MS medium with various concentrations of 6-benzyladenine (BA) and α-naphthaleneacetic acid (NAA). The highest numbers of adventitious buds were obtained on MS medium supplemented with 10 μM BA and 1 μM NAA. The replacement of 7 g l-1 Difco bacto agar with 2.6 g l-1 Phytagel in the medium enhanced adventitious bud regeneration. Further, addition of 15 μM silver nitrate promoted callus-free shoot regeneration from leaf explants. The regenerated shoot buds were elongated on MS medium containing 0.01 μM BA and 1 μM NAA. Rooting was obtained on modified MS medium supplemented with 2 μM IBA. To our knowledge this is the first report of direct regeneration of shoots from leaflet explants in A. procera, and should help facilitate genetic transformation in this species.