Morphological features and lipopolysaccharide attachment of coliphages specific to Escherichia coli O157:H7 and to a broad range of E. coli hosts

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• 作者：Eun-Jin Kim ; Heyn Lee ; Ju-Hoon Lee ; Sangryol Ryu…
• 关键词：Bacteriophage ; E. coli O157 ; H7 ; Tail ; Receptor ; Lipopolysaccharide
• 刊名：Journal of the Korean Society for Applied Biological Chemistry
• 出版年：2016
• 出版时间：February 2016
• 年：2016
• 卷：59
• 期：1
• 页码：109-116
• 全文大小：3,092 KB
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• 作者单位：Eun-Jin Kim (1)
  Heyn Lee (2)
  Ju-Hoon Lee (2)
  Sangryol Ryu (3)
  Jong-Hyun Park (1)
  
  1. Department of Food Science and Biotechnology, Gachon University, Sungnam, 13120, Republic of Korea
  2. Department of Food Science and Biotechnology, Institute of Life Sciences and Resources, Kyung Hee University, Yongin, 17104, Republic of Korea
  3. Department of Food and Animal Biotechnology, Seoul National University, Seoul, 08826, Republic of Korea
  
• 刊物主题：Applied Microbiology; Bioorganic Chemistry; Biological Techniques;
• 出版者：Springer Netherlands
• ISSN：2234-344X

文摘

The objective of the present study was to analyze host-phage adsorption of bacteriophages infecting Escherichia coli O157:H7 and the other E. coli strains. Out of 55 coliphage strains, we selected seven coliphages infectious only to 23 E. coli O157 and seven other coliphages of broad specificity to E. coli O157:H7 and other 61 E. coli. Escherichia coli O157-specific phages and the broadly specific phages all belonged to the Siphoviridae and Myoviridae family, respectively. Escherichia coli O157-specific phages infected E. coli O157:H7, but not E. coli O157:H7△rfaL, deletion mutant of O-antigen ligase gene for lipopolysaccharide. Five coliphages among the broadly specific phages infected E. coli O103, but not E. coli O103△rfaG, deletion mutant of the glycosyltransferase gene. E. coli O157:H7-specific phages among Siphoviridae recognized O-antigen of E. coli O157, but the broadly specific coliphages of Myoviridae may recognize O-antigen and/or a part of the lipopolysaccharide core as an adsorption site in various E. coli. The receptor of the two coliphage groups interacts with some part of lipopolysaccharide, and the tail morphology of the coliphages may be related to their adsorption to and recognition of a different part of lipopolysaccharide. In particular, specificity of E. coli O157:H7-specific phages carrying the long tail of Siphoviridae for O-antigen as a receptor seems to be high.