Gene expression analysis reveals a functional role for the Ag-NPs-induced Egr-1 transcriptional factor in human keratinocytes

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• 作者：Gyeongmin Yoo (1)
  Sang Hoon Jeong (1)
  Woo In Ryu (1)
  Hana Lee (1)
  Jin Hee Kim (1)
  Hyun Cheol Bae (1)
  Sang Wook Son (1)
  
• 关键词：Silver nanoparticles ; Early growth response ; 1 ; Microarray ; Keratinocytes
• 刊名：Molecular & Cellular Toxicology
• 出版年：2014
• 出版时间：June 2014
• 年：2014
• 卷：10
• 期：2
• 页码：149-156
• 全文大小：316 KB
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• 作者单位：Gyeongmin Yoo (1)
  Sang Hoon Jeong (1)
  Woo In Ryu (1)
  Hana Lee (1)
  Jin Hee Kim (1)
  Hyun Cheol Bae (1)
  Sang Wook Son (1)
  
  1. Laboratory of Cell Signaling and Nanomedicine, Department of Dermatology and Division of Brain Korea 21 Project for Biomedical Science, Korea University College of Medicine, Seoul, Korea
  
• ISSN：2092-8467

文摘

Silver nanoparticles (Ag-NPs) have wellknown antimicrobial properties and have been widely applied as nano-strategies in myriad medical fields. Indeed, early growth response (Egr-1) is an important transcription factor that has been implicated in numerous inflammatory diseases. The administration of Ag-NPs transiently increased the levels of Egr-1 mRNA in human keratinocytes with maximal induction at the 30 minute time point. In an effort to identify molecular signatures associated with cellular responses to Ag-NPs, we adopted an approach involving gene expression profiling of human keratinocytes treated with or without Egr-1 siRNA transfection using a cDNA microarray. The microarray analysis demonstrated that: the expression profile of the genes involved in inflammation and immune response were either stimulated or repressed. Overall, this study was sufficient to reliably recognize the engagement of Egr-1-driven molecular signaling pathways in Ag-NPs-treated keratinocytes.