Sarcocystis spp. in sheep and goats: frequency of infection and species identification by morphological, ultrastructural, and molecular tests in Bahia, Brazil

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• 作者：Marta Vasconcelos Bittencourt ; Iris Daniela S. Meneses…
• 关键词：Sarcocystis ; Goat ; Sheep ; Ultrastructure ; Molecular
• 刊名：Parasitology Research
• 出版年：2016
• 出版时间：April 2016
• 年：2016
• 卷：115
• 期：4
• 页码：1683-1689
• 全文大小：546 KB
• 参考文献：Al Quraishy S, Morsy K, Bashtar AR, Ghaffar FA, Mehlhorn H (2014) Sarcocystis arieticanis (Apicomplexa: Sarcocystidae) infecting the heart muscles of the domestic sheep, Ovis aries (Artiodactyla: Bovidae), from K. S. A. on the basis of light and electron microscopic data. Parasitol Res doi:10.​1007/​s00436-014-4050-2
  Buxton D (1998) Protozoan infections (Toxoplasma gondii, Neospora caninum and Sarcocystis spp.) in sheep and goats: recent advances. Vet Res 29:289–310PubMed
  Collins GH, Atkinson E, Charleston WA (1979) Studies on Sarcocystis species III: the macrocystic species of sheep. N Z Vet J 27:204–206CrossRef PubMed
  da Silva RC, Su C, Langoni H (2009) First identification of Sarcocystis tenella (Railliet, 1886) Moule, 1886 (Protozoa: Apicomplexa) by PCR in naturally infected sheep from Brazil. Vet Parasitol 165:332–336. doi:10.​1016/​j.​vetpar.​2009.​07.​016 CrossRef PubMed
  Dahlgren SS, Gjerde B (2007) Genetic characterisation of six Sarcocystis species from reindeer (Rangifer tarandus tarandus) in Norway based on the small subunit rRNA gene. Vet Parasitol 146:204–213. doi:10.​1016/​j.​vetpar.​2007.​02.​023 CrossRef PubMed
  Dubey JP, Livingston CW Jr (1986) Sarcocystis capracanis and Toxoplasma gondii infections in range goats from Texas. Am J Vet Res 47:523–524PubMed
  Dubey JP, Speer CA, Epling GP (1982) Sarcocystosis in newborn calves fed Sarcocystis cruzi sporocysts from coyotes. Am J Vet Res 43:2147–2164PubMed
  Dubey JP, Lindsay DS, Speer CA, Fayer R, Livingston CW Jr (1988) Sarcocystis arieticanis and other Sarcocystis species in sheep in the United States. J Parasitol 74:1033–1038CrossRef PubMed
  Dubey JP, Speer CA, Fayer R (1989) Sarcocystosis of animals and man. CRC Press, Boca Raton
  Ellis J, Morrison D (1995) Effects of sequence alignment on the phylogeny of Sarcocystis deduced from 18S rDNA sequences. Parasitol Res 81:696–699CrossRef PubMed
  Ellis TJ, Luton K, Baverstock PR, Whitworth G, Tenter AM, Johnson AM (1995) Phylogenetic relationships between Toxoplasma and Sarcocystis deduced from a comparison of 18S rDNA sequences. Parasitology 110(Pt 5):521–528CrossRef PubMed
  Fischer S, Odening K (1998) Characterization of bovine Sarcocystis species by analysis of their 18S ribosomal DNA sequences. J Parasitol 84:50–54CrossRef PubMed
  Gjerde B (2013) Phylogenetic relationships among Sarcocystis species in cervids, cattle and sheep inferred from the mitochondrial cytochrome c oxidase subunit I gene. Int J Parasitol 43:579–591. doi:10.​1016/​j.​ijpara.​2013.​02.​004S0020-7519(13)00087-8 CrossRef PubMed
  Haziroglu R, Guvenc T, Tunca R (2003) Electron microscopical studies on cysts of Sarcocystis arieticanis within cardiac muscle of naturally infected sheep. Parasitol Res 89:23–25. doi:10.​1007/​s00436-001-0549-4 PubMed
  Heydorn AO, Haralambidis S (1982) Development of Sarcocystis capracanis Fischer, 1979. Berl Munch Tierarztl Wochenschr 95:265–271PubMed
  Heydorn AO, Kirmsse P (1996) Isolation and experimental transmission of Sarcocystis moulei Neveu-Lemaire, 1912. Berl Munch Tierarztl Wochenschr 109:440–445PubMed
  Heydorn AO, Matuschka FR (1981) Final host specificity of Sarcocystis species transmitted by dogs (authors’s transl). Z Parasitenkd 66:231–234CrossRef PubMed
  Heydorn AO, Mehlhorn H (1987) Fine structure of Sarcocystis arieticanis Heydorn, 1985 in its intermediate and final hosts (sheep and dog). Zentralbl Bakteriol Mikrobiol Hyg A 264:353–362PubMed
  Heydorn AO, Gestrich R, Mehlhorn H, Rommel M (1975) Proposal for a new nomenclature of the Sarcosporidia. Z Parasitenkd 48:73–82CrossRef PubMed
  Holmdahl OJ, Mattsson JG, Uggla A, Johansson KE (1993) Oligonucleotide probes complementary to variable regions of 18S rRNA from Sarcocystis species. Mol Cell Probes 7:481–486. doi:10.​1006/​mcpr.​1993.​1071 CrossRef PubMed
  Instituto Brasileiro de Geografia e Estatística—IBGE (2011) Produção da pecuária municipal. Rio de Janeiro 39:1–63
  Jenkins MC, Ellis JT, Liddell S, Ryce C, Munday BL, Morrison DA, Dubey JP (1999) The relationship of Hammondia hammondi and Sarcocystis mucosa to other heteroxenous cyst-forming coccidia as inferred by phylogenetic analysis of the 18S SSU ribosomal DNA sequence. Parasitology 119(Pt 2):135–142CrossRef PubMed
  Latif BM, Al-Delemi JK, Mohammed BS, Al-Bayati SM, Al-Amiry AM (1999) Prevalence of Sarcocystis spp. in meat-producing animals in Iraq. Vet Parasitol 84:85–90CrossRef PubMed
  Levine ND (1986) The taxonomy of Sarcocystis (Protozoa, Apicomplexa) species. J Parasitol 72:372–382CrossRef PubMed
  Martínez-Navalón B et al (2012) Sarcocystis infection: a major cause of carcass condemnation in adult sheep in Spain. Span J Agric Res 10:388–392. doi:10.​5424/​sjar/​2012102-523-11 CrossRef
  Mehlhorn H, Scholtyseck E (1974) Light and electron microscope studies on stages of Sarcocystis tenella in the intestine of cats. I. The oocysts and sporocysts (author’s transl). Z Parasitenkd 43:251–270CrossRef PubMed
  Morsy K et al (2011) Prevalence pattern and biology of Sarcocystis capracanis infection in the Egyptian goats: a light and ultrastructural study. Vet Parasitol 181:75–82. doi:10.​1016/​j.​vetpar.​2011.​05.​010 CrossRef PubMed
  Munday BL, Obendorf DL (1984) Development and growth of Sarcocystis gigantea in experimentally-infected sheep. Vet Parasitol 15:203–211CrossRef PubMed
  Munday BL, Rickard MD (1974) Is Sarcocystis tenella two species. Aust Vet J 50:558–559CrossRef PubMed
  Obendorf DL, Munday BL (1987) Experimental infection with Sarcocystis medusiformis in sheep. Vet Parasitol 24:59–65CrossRef PubMed
  Oryan A, Moghaddar N, Gaur SN (1996) The distribution pattern of Sarcocystis species, their transmission and pathogenesis in sheep in Fars Province of Iran. Vet Res Commun 20:243–253CrossRef PubMed
  Pescador CA, Corbellini LG, Oliveira EC, Bandarra PM, Leal JS, Pedroso PMO, Dremeier D (2007) Aborto ovino associado com infecção por Sarcocystis sp. Pesqui Vet Bras 27:393–397
  Shekarforoush SS, Razavi SM, Dehghan SA, Sarihi K (2005) Prevalence of Sarcocystis species in slaughtered goats in Shiraz. Iran Vet Rec 156:418–420CrossRef PubMed
  Singh KP, Agrawal MC, Shah HL (1990) Prevalence of sarcocysts of Sarcocystis capracanis in oesophagus and tail muscles of naturally infected goats. Vet Parasitol 36:153–155CrossRef PubMed
  Yang ZQ, Zuo YX, Ding B, Chen XW, Luo J, Zhang YP (2001a) Identification of Sarcocystis hominis-like (Protozoa: Sarcocystidae) cyst in water buffalo (Bubalus bubalis) based on 18S rRNA gene sequences. J Parasitol 87:934–937. doi:10.​1645/​0022-3395(2001)087[0934:​IOSHLP]2.​0.​CO;2 CrossRef PubMed
  Yang ZQ, Zuo YX, Yao YG, Chen XW, Yang GC, Zhang YP (2001b) Analysis of the 18S rRNA genes of Sarcocystis species suggests that the morphologically similar organisms from cattle and water buffalo should be considered the same species. Mol Biochem Parasitol 115:283–288CrossRef PubMed
  
• 作者单位：Marta Vasconcelos Bittencourt (1)
  Iris Daniela S. Meneses (1)
  Müller Ribeiro-Andrade (1)
  Rogério Fernando de Jesus (1)
  Flábio Ribeiro de Araújo (2)
  Luís F. Pita Gondim (1)
  
  1. Escola de Medicina Veterinária e Zootecnia, Universidade Federal da Bahia, Av. Adhemar de Barros 500, Ondina, CEP 40170-110, Salvador, Bahia, Brazil
  2. Embrapa Gado de Corte, Avenida Rádio Maia 830, 79106-550, Campo Grande, MS, Brazil
  
• 刊物类别：Biomedical and Life Sciences
• 刊物主题：Biomedicine
  Medical Microbiology
  Microbiology
  Immunology
  
• 出版者：Springer Berlin / Heidelberg
• ISSN：1432-1955

文摘

Sarcocystis spp. are cyst-forming coccidia that infect numerous animals species, including several livestock species. Despite the importance of sheep and goat production in Brazil, little it is known about the Sarcocystis species that infect small ruminants in the country and their potential impact on meat condemnation due to the presence of macroscopic cysts of the parasite. The aims of the present study were to determine the frequency of infection by Sarcocystis spp. in goats and sheep intended for human consumption in Bahia State, Brazil, as well as to identify the parasite species in selected samples. The entire tongue, esophagus, and heart were collected from 120 goats and 120 sheep. Tissues were examined for Sarcocystis spp. by macroscopic evaluation, light microscopy, electron microscopy, and molecular tests. Microscopic cysts of Sarcocystis spp. were detected in 95.8 % of sheep and 91.6 % of goats. Using either transmission electron microscopy or partial sequencing of the 18S region of the ribosomal DNA (rDNA) for species identification, Sarcocystis tenella and Sarcocystis arieticanis were observed in sheep and Sarcocystis capracanis in goats. Macroscopic cysts were not detected in the analyzed samples. We concluded that goats and sheep destined for human consumption in Bahia possess high frequencies of Sarcocystis infection. Carcass condemnation due to Sarcocystis macrocysts seems to be rare in the studied region. S. arieticanis and S. capracanis were confirmed for the first time by electron microscopy or by molecular tests in small ruminants from Brazil.