Rapid immunohistochemistry based on alternating current electric field for intraoperative diagnosis of brain tumors

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• 作者：Mishie Tanino ; Toshio Sasajima ; Hiroshi Nanjo ; Shiori Akesaka…
• 关键词：Rapid immunohistochemistry (R ; IHC) ; Glioma ; Central nervous system ; lymphoma (CNS ; lymphoma)
• 刊名：Brain Tumor Pathology
• 出版年：2015
• 出版时间：January 2015
• 年：2015
• 卷：32
• 期：1
• 页码：12-19
• 全文大小：1,526 KB
• 参考文献：1. Brat DJ, Prayson RA, Ryken TC et al (2008) Diagnosis of malignant glioma: role of neuropathology. J Neurooncol 89(3):287-11 CrossRef
  2. Faehndrich J, Weidauer S, Pilatus U et al (2011) Neuroradiological viewpoint on the diagnostics of space-occupying brain lesions. Clin Neuroradiol 21(3):123-39 CrossRef
  3. Nihashi T, Dahabreh IJ, Terasawa T (2013) Diagnostic accuracy of PET for recurrent glioma diagnosis: a meta-analysis. AJNR 34(5):944-50 S1–S11 CrossRef
  4. Louis DN, Ohgaki H, Wiestler OD et al (2007) WHO classification of tumors of the central nervous system. IARC, Lyon
  5. Plesec TP, Prayson RA (2007) Frozen section discrepancy in the evaluation of central nervous system tumors. Arch Pathol Lab Med 131(10):1532-540
  6. Uematsu Y, Owai Y, Okita R et al (2007) The usefulness and problem of intraoperative rapid diagnosis in surgical neuropathology. Brain Tumor Pathol 24(2):47-2 CrossRef
  7. Ichihara T, Nakao A, Suzuki Y et al (1989) Improvement of the rapid immunoperoxidase staining method for intraoperative pathological diagnosis of pancreatic cancer using microwave irradiation. J Surg Oncol 42(3):209-14 CrossRef
  8. Richter T, Nahrig J, Komminoth P et al (1999) Protocol for ultrarapid immunostaining of frozen sections. J Clin Pathol 52(6):461-63 CrossRef
  9. Kammerer U, Kapp M, Gassel AM et al (2001) A new rapid immunohistochemical staining technique using the EnVision antibody complex. J Histochem Cytochem 49(5):623-30 CrossRef
  10. Haapasalo J, Mennander A, Helen P et al (2005) Ultrarapid Ki-67 immunostaining in frozen section interpretation of gliomas. J Clin Pathol 58(3):263-68 CrossRef
  11. Uzuka T, Aoki H, Natsumeda M et al (2011) Indication of intraoperative immunohistochemistry for accurate pathological diagnosis of brain tumors. Brain Tumor Pathol 28(3):239-46 CrossRef
  12. Monig SP, Luebke T, Soheili A et al (2006) Rapid immunohistochemical detection of tumor cells in gastric carcinoma. Oncol Rep 16(5):1143-147
  13. Hatta H, Tsuneyama K, Kumada T et al (2006) Freshly prepared immune complexes with intermittent microwave irradiation result in rapid and high-quality immunostaining. Pathol Res Pract 202(6):439-45 CrossRef
  14. Hatta H, Tsuneyama K, Kondo T et al (2010) Development of an ultrasound-emitting device for performing rapid immunostaining procedures. J Histochem Cytochem 58(5):421-28 CrossRef
  15. Toda H, Minamiya Y, Kagaya M et al (2011) A novel immunohistochemical staining method allows ultrarapid detection of lymph node micrometastases while conserving antibody. Acta Histochem Cytochem 44(3):133-39 CrossRef
  16. Firlik KS, Martinez AJ, Lunsford LD (1999) Use of cytological preparations for the intraoperative diagnosis of stereotactically obtained brain biopsies: a 19-year experience and survey of neuropathologists. J Neurosurg 91(3):454-58 CrossRef
  
• 作者单位：Mishie Tanino (1)
  Toshio Sasajima (2)
  Hiroshi Nanjo (3)
  Shiori Akesaka (1)
  Masami Kagaya (4)
  Taichi Kimura (1)
  Yusuke Ishida (1)
  Masaya Oda (2)
  Masataka Takahashi (2)
  Taku Sugawara (2)
  Toshiaki Yoshioka (3)
  Hiroshi Nishihara (6)
  Yoichi Akagami (4)
  Akiteru Goto (3)
  Yoshihiro Minamiya (5)
  Shinya Tanaka (1) (6)
  R-IHC Study Group
  
  1. Department of Cancer Pathology, Hokkaido University Graduate School of Medicine, N15, W7, Kita-ku, Sapporo, 060-8638, Japan
  2. Department of Neurosurgery, Akita University Graduate School of Medicine, 1-1-1 Hondo, Akita, 010-8543, Japan
  3. Division of Clinical Pathology, Akita University Graduate School of Medicine, 1-1-1 Hondo, Akita, 010-8543, Japan
  4. Akita Industry Technology Center, Sanuki, Aza, Arayamachi, Akita, 010-1623, Japan
  6. Department of Translational Pathology, Hokkaido University Graduate School of Medicine, N15W7, Kita-ku, Sapporo, 060-8638, Japan
  5. Department of Thoracic Surgery, Akita University Graduate School of Medicine, 1-1-1 Hondo, Akita, 010-8543, Japan
  
• 刊物主题：Neurosurgery; Neurology; Pathology; Oncology; Cancer Research;
• 出版者：Springer Japan
• ISSN：1861-387X

文摘

Rapid immunohistochemistry (R-IHC) can contribute to the intraoperative diagnosis of central nervous system (CNS) tumors. We have recently developed a new IHC method based on an alternating current electric field to facilitate the antigen–antibody reaction. To ensure the requirement of R-IHC for intraoperative diagnosis, 183 cases of CNS tumors were reviewed regarding the accuracy rate of diagnosis without R-IHC. The diagnostic accuracy was 90.7?% (168/183 cases) in which definitive diagnoses were not provided in 17 cases because of the failure of glioma grading and differential diagnosis of lymphoma and glioma. To establish the clinicopathological application, R-IHC for frozen specimens was compared with standard IHC for permanent specimens. 33 gliomas were analyzed, and the Ki-67/MIB-1 indices of frozen specimens by R-IHC were consistent with the grade and statistically correlated with those of permanent specimens. Thus, R-IHC provided supportive information to determine the grade of glioma. For discrimination between glioma and lymphoma, R-IHC was able to provide clear results of CD20 and Ki-67/MIB-1 in four frozen specimens of CNS lymphoma as well as standard IHC. We conclude that the R-IHC for frozen specimens can provide important information for intraoperative diagnosis of CNS tumors.