Enzymatic activities for lignin monomer intermediates highlight the biosynthetic pathway of syringyl monomers in Robinia pseudoacacia
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- 作者：Jun Shigeto ; Yukie Ueda ; Shinya Sasaki ; Koki Fujita…
- 关键词：4 ; Coumarate ; coenzyme A ligase (4CL) ; Lignin biosynthesis ; Robinia pseudoacacia ; Sinapic acid ; Syringyl lignin
- 刊名：Journal of Plant Research
- 出版年：2017
- 出版时间：January 2017
- 年：2017
- 卷：130
- 期：1
- 页码：203-210
- 全文大小：
- 刊物类别：Biomedical and Life Sciences
- 刊物主题：Plant Sciences; Plant Ecology; Plant Physiology; Plant Biochemistry;
- 出版者：Springer Japan
- ISSN：1618-0860
- 卷排序：130
文摘
Most of the known 4-coumarate:coenzyme A ligase (4CL) isoforms lack CoA-ligation activity for sinapic acid. Therefore, there is some doubt as to whether sinapic acid contributes to sinapyl alcohol biosynthesis. In this study, we characterized the enzyme activity of a protein mixture extracted from the developing xylem of Robinia pseudoacacia. The crude protein mixture contained at least two 4CLs with sinapic acid 4-CoA ligation activity. The crude enzyme preparation displayed negligible sinapaldehyde dehydrogenase activity, but showed ferulic acid 5-hydroxylation activity and 5-hydroxyferulic acid O-methyltransferase activity; these activities were retained in the presence of competitive substrates (coniferaldehyde and 5-hydroxyconiferaldehyde, respectively). 5-Hydroxyferulic acid and sinapic acid accumulated in the developing xylem of R. pseudoacacia, suggesting, in part at least, sinapic acid is a sinapyl alcohol precursor in this species.