Use of droplet digital PCR for quantitative and automatic analysis of the HER2 status in breast cancer patients

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• 作者：Kazutaka Otsuji ; Takeshi Sasaki ; Atsushi Tanaka…
• 关键词：Breast cancer ; HER2 ; Digital PCR ; Gene amplification ; Tumor content ratio
• 刊名：Breast Cancer Research and Treatment
• 出版年：2017
• 出版时间：February 2017
• 年：2017
• 卷：162
• 期：1
• 页码：11-18
• 全文大小：
• 刊物类别：Medicine
• 刊物主题：Oncology;
• 出版者：Springer US
• ISSN：1573-7217
• 卷排序：162

文摘

PurposeDigital polymerase chain reaction (dPCR) has been used to yield an absolute measure of nucleic acid concentrations. Recently, a new method referred to as droplet digital PCR (ddPCR) has gained attention as a more precise and less subjective assay to quantify DNA amplification. We demonstrated the usefulness of ddPCR to determine HER2 gene amplification of breast cancer.