Detection of satellite cells during skeletal muscle wound healing in rats: time-dependent expressions of Pax7 and MyoD in relation to wound age
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- 作者：Zhi-Ling Tian ; Shu-Kun Jiang ; Miao Zhang…
- 关键词：Wound age estimation ; Skeletal muscle contusion ; Satellite cell ; Pax7 ; MyoD
- 刊名：International Journal of Legal Medicine
- 出版年：2016
- 出版时间：January 2016
- 年：2016
- 卷：130
- 期：1
- 页码：163-172
- 全文大小：3,946 KB
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Shu-Kun Jiang (1)
Miao Zhang (1)
Meng Wang (1)
Jiao-Yong Li (1)
Rui Zhao (1)
Lin-Lin Wang (1)
Shan-Shan Li (1)
Min Liu (1)
Meng-Zhou Zhang (1)
Da-Wei Guan (1)
1. Department of Forensic Pathology, China Medical University School of Forensic Medicine, No.77, Puhe Road, Shenbei New Area, Shenyang, 110122, Liaoning Province, People’s Republic of China - 刊物类别：Medicine
- 刊物主题：Medicine & Public Health
Forensic Medicine
Medical Law
Medicine/Public Health, general - 出版者：Springer Berlin / Heidelberg
- ISSN：1437-1596
文摘
The study was focused on time-dependent expressions of paired-box transcription factor 7 (Pax7) and myoblast determination protein (MyoD) during skeletal muscle wound healing. An animal model of skeletal muscle contusion was established in 40 Sprague–Dawley male rats. Samples were taken at 1, 3, 5, 7, 9, 13, 17, and 21 days after injury, respectively (five rats in each posttraumatic interval). Five rats were employed as control. By morphometric analysis, the data based on the number of Pax7+/MyoD−, Pax7+/MyoD+, and Pax7−/MyoD+ cells were highly correlated with the wound age. Pax7 and MyoD expressions were upregulated after injury by Western blot and quantitative real-time PCR assays. The relative quantity of Pax7 protein peaked at 5 days after injury, which was >1.13, and decreased thereafter. Similarly, the relative quantity of MyoD mRNA expression peaked at 3 days after injury, which was >2.59. The relative quantity of Pax7 protein >0.73 or mRNA expression >2.38 or the relative quantity of MyoD protein >1.33 suggested a wound age of 3 to 7 days. The relative quantity of MyoD mRNA expression >2.02 suggested a wound age of 1 to 7 days post-injury. In conclusion, the expressions of Pax7 and MyoD are upregulated in a time-dependent manner during skeletal muscle wound healing, suggesting that Pax7 and MyoD may be potential markers for wound age estimation in skeletal muscle. Keywords Wound age estimation Skeletal muscle contusion Satellite cell Pax7 MyoD