Induced Homoeologous Pairing for Transfer of Useful Variability for High Grain Fe and Zn from Aegilops kotschyi into Wheat

详细信息    查看全文

• 作者：Shailender Kumar Verma ; Satish Kumar ; Imran Sheikh…
• 关键词：Aegilops ; Wheat ; Iron ; Zinc ; Chromosome 5B ; Homoeologous recombination
• 刊名：Plant Molecular Biology Reporter
• 出版年：2016
• 出版时间：December 2016
• 年：2016
• 卷：34
• 期：6
• 页码：1083-1094
• 全文大小：2,569 KB
• 刊物类别：Biomedical and Life Sciences
• 刊物主题：Life Sciences
  Plant Sciences
  Plant Physiology
  
• 出版者：Springer Netherlands
• ISSN：1572-9818
• 卷排序：34

文摘

Biofortification of bread wheat by the transfer of useful variability of high grain Fe and Zn from Aegilops kotschyi through induced homoeologous pairing is the most feasible approach to alleviate micronutrient malnutrition worldwide. Deficiency of chromosome 5B in interspecific hybrids allows homoeologous pairing and recombination of chromosomes of wheat with those of the related species. The interspecific hybrid plants without 5B chromosome showed much higher chromosome pairing than did the plants with 5B. The F₁ plants without 5B chromosome were selected and repeatedly backcrossed with wheat cultivar PBW343. The chromosome number of BC₂F₁ plants ranged from 43 to 60 with several univalents and multivalents. Molecular markers and GISH analysis confirmed the introgression of U/S chromosomes of Ae. kotschyi and their fragments in wheat. The BC₂F₂ plants showed up to 125 % increase in Fe and 158 % increase in Zn compared to PBW343 with Lr24 and Yr36. Induced homoeologous pairing in the absence of 5B was found to be an effective approach for transfer of useful variability for enhanced grain Fe and Zn content for biofortification of wheat for high grain micronutrient content.