Cloning and functional analysis of pale-green leaf (PGL10) in rice (Oryza sativa L.)

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• 作者：Yao-Long Yang ; Jie Xu ; Yu-Chun Rao ; Yong-Jun Zeng ; Hui-Juan Liu…
• 关键词：Rice (Oryza sativa L.) ; Pale ; green leaf (PGL10) ; Map ; based cloning
• 刊名：Plant Growth Regulation
• 出版年：2016
• 出版时间：January 2016
• 年：2016
• 卷：78
• 期：1
• 页码：69-77
• 全文大小：908 KB
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• 作者单位：Yao-Long Yang (1) (2)
  Jie Xu (1) (2)
  Yu-Chun Rao (3)
  Yong-Jun Zeng (1)
  Hui-Juan Liu (2)
  Ting-Ting Zheng (2)
  Guang-Heng Zhang (2)
  Jiang Hu (2)
  Long-Biao Guo (2)
  Qian Qian (2)
  Da-Li Zeng (2)
  Qing-Hua Shi (1)
  
  1. Key Laboratory of Crop Physiology, Ecology and Genetic Breeding, Ministry of Education, Jiangxi Agricultural University, Nanchang, 330045, People’s Republic of China
  2. State Key Lab for Rice Biology, China National Rice Research Institute, Hangzhou, 310006, People’s Republic of China
  3. College of Chemistry and Life Sciences, Zhejiang Normal University, Jinhua, 321004, People’s Republic of China
  
• 刊物类别：Biomedical and Life Sciences
• 刊物主题：Life Sciences
  Plant Physiology
  
• 出版者：Springer Netherlands
• ISSN：1573-5087

文摘

Leaf color mutants commonly found in rice have important implications in basic research and breeding science. In this study, we isolated a pale-green leaf mutant (pgl10) from the offspring of the rice cultivar Nipponbare (Oryza sativa L. spp. japonica) through ethyl methanesulfonate mutagenesis. Compared with the wild-type Nipponbare, the pgl10 mutant had phenotypically pale-green leaves with significantly decreased chlorophyll (a and b) and carotenoid contents. Transmission electron micrographs showed that pgl10 had less grana lamellae of chloroplasts than Nipponbare. The results of tissue-specific gene expression analysis revealed that pgl10 was expressed in various rice organs, including roots, stems, leaves, sheaths, and spikes. The expression of Chl synthesis-associated gene in pgl10 was decreased. Genetic analysis suggested that PGL10 was controlled by a recessive gene. Map-based cloning and genome sequencing data showed that pgl10 was a frameshift mutation caused by a single base insertion on chromosome 10. Bioinformation analysis indicated that PGL10 encoded protochlorophyllide oxidoreductase B. Therefore, pgl10 can be a genetic material for further studies on PGL10. Keywords Rice (Oryza sativa L.) Pale-green leaf (PGL10) Map-based cloning