Transformation of Saussurea medusa for hairy roots and jaceosidin production

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• 作者：Dexiu Zhao ; Chunxiang Fu ; Yaqiong Chen and Fengshan Ma
• 关键词：Agrobacterium rhizogenes ; Jaceosidin ; Saussurea medusa ; Hairy roots
• 刊名：Plant Cell Reports
• 出版年：2004
• 出版时间：December 2004
• 年：2004
• 卷：23
• 期：7
• 页码：468-474
• 全文大小：267 KB
• 刊物类别：Biomedical and Life Sciences
• 刊物主题：Life Sciences
  Cell Biology
  Plant Sciences
  Biotechnology
  Plant Biochemistry
  
• 出版者：Springer Berlin / Heidelberg
• ISSN：1432-203X

文摘

Axenically grown Saussurea medusa plantlets were inoculated with four Agrobacterium rhizogenes strains, and hairy root lines were established with A. rhizogenes strain R1601 in N6 medium. PCR and Southern hybridization confirmed integration of the T-DNA fragment of the Ri plasmid from A. rhizogenes into the genome of S. medusa hairy roots. In N6 medium, maximum biomass of the hairy root cultures was achieved [8 g (dry weight) per liter; growth ratio 35-fold] after 21 days of culture. The amount of jaceosidin extracted from the hairy root cultures was 46 mg/l (production ratio of 37-fold) after 27 days of culture. The maximum jaceosidin content obtained using N6 medium was higher than that obtained with Modified White, MS or B5 medium. In N6 medium, the tip segments were more efficient for hairy root growth and jaceosidin production than the middle and basal regions of the root.