Characterization of Anthocyanidin Synthase (ANS) Gene and anthocyanidin in rare medicinal plant- Saussurea medusa

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• 作者：Liqin Cheng ; Yanjun Xu ; Erich Grotewold ; Zhiping Jin ; Fengyan Wu ; Chunxiang Fu and Dexiu Zhao
• 关键词：Anthocyanins ; Expression patterns ; HPLC ; ESI ; MS ; Saussurea medusa
• 刊名：Plant Cell, Tissue and Organ Culture
• 出版年：2007
• 出版时间：April, 2007
• 年：2007
• 卷：89
• 期：1
• 页码：63-73
• 全文大小：676 KB
• 刊物类别：Biomedical and Life Sciences
• 刊物主题：Life Sciences
  Plant Sciences
  Plant Physiology
  
• 出版者：Springer Netherlands
• ISSN：1573-5044

文摘

Anthocyanidin synthase (ANS, leucoanthocyanidin oxygenase), a 2-oxoglutarate iron-dependent oxygenase, catalyzes the penultimate step in the biosynthesis of the anthocyanin class of flavonoids, from the colorless leucoanthocyanidins to the colored anthocyanidins. Here, we report the cloning and characterization of an anthocyanidin Synthase gene (SmANS1) from a cDNA library derived from a red callus line of Saussurea medusa, a traditional Chinese medicinal plant. SmANS1 exhibits significant similarities in amino acid sequence with members of a family of 2-oxoglutarate-dependent oxygenases. The deduced SmANS1 protein shared 83% and 79% identity with that of Callistephus chinensis and Gerbera hybrids, respectively. Reverse transcription-PCR (RT-PCR) analyses of SmANS1 transcripts showed that the SmANS1 was abundantly expressed in the cell cultures of red cell line and seedlings, and weakly expressed in the counterparts of yellow cell line, but was not expressed in the roots. Further more, the anthocyanins composition of cell cultures from Saussurea medusa was investigated using high-performance liquid chromatography/mass spectrometry (HPLC-ESI-MS) and tandem mass spectrometry (MS-MS). Major anthocyanins were identified as 3-O-glucosides cyanidin derivatives and minor peaks were identified as pelargonidin derivatives.