Biological toxicity of cellulose nanocrystals (CNCs) against the luxCDABE-based bioluminescent bioreporter Escherichia coli 652T7

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• 作者：Liyu Du ; Kelly Arnholt ; Steven Ripp ; Gary Sayler ; Siqun Wang…
• 关键词：Cellulose nanocrystals ; Toxicity ; Lux ; Bioreporter ; Bioluminescence
• 刊名：Ecotoxicology
• 出版年：2015
• 出版时间：December 2015
• 年：2015
• 卷：24
• 期：10
• 页码：2049-2053
• 全文大小：606 KB
• 参考文献：Bondeson D, Mathew A, Oksman K (2006) Optimization of the isolation of nanocrystals from microcrystalline cellulose by acid hydrolysis. Cellulose 13:171鈥?80CrossRef
  Cundy AB, Hopkinson L, Whitby RLD (2008) Use of iron-based technologies in contaminated land and groundwater remediation: a review. Sci Total Environ 400:42鈥?1CrossRef
  Donaldson K, Poland CA (2013) Nanotoxicity: challenging the myth of nano-specific toxicity. Curr Opin Biotechnol 24:724鈥?34CrossRef
  Espino-Perez E, Domenek S, Belgacem N, Sillard C, Bras J (2014) Green process for chemical functionalization of nanocellulose with carboxylic acids. Biomacromolecules 15:4551鈥?560CrossRef
  Faedmaleki F, Shirazi FH, Salarian A-A, Ashtiani HA, Rastegar H (2014) Toxicity effect of silver nanoparticles on mice liver primary cell culture and HepG(2) cell line. Iran J Pharm Res 13:235鈥?42
  Gerber C, Lang HP (2006) How the doors to the nanoworld were opened. Nat Nanotechnol 1:3鈥?CrossRef
  Jia H, Wang Z, Wang C, Chang L, Li Z (2014a) Real-time fluorescence detection of Hg2+ ions with high sensitivity by exponentially isothermal oligonucleotide amplification. RSC Adv 4:9439鈥?444CrossRef
  Jia K, Marks RS, Ionescu RE (2014b) Influence of carbon-based nanomaterials on lux-bioreporter Escherichia coli. Talanta 126:208鈥?13CrossRef
  Kang JL, Moon C, Lee HS, Lee HW, Park E-M, Kim HS, Castranova V (2008) Comparison of the biological activity between ultrafine and fine titanium dioxide particles in RAW 264.7 cells associated with oxidative stress. J Toxicol Environ Health Part A 71:478鈥?85CrossRef
  Kasemets K, Ivask A, Dubourguier HC, Kahru A (2009) Toxicity of nanoparticles of ZnO, CuO and TiO2 to yeast Saccharomyces cerevisiae. Toxicol Vitro 23:1116鈥?122CrossRef
  Lee JH, Lee J-Y, Yang SH, Lee E-J, Kim H-W (2014) Carbon nanotube-collagen three-dimensional culture of mesenchymal stem cells promotes expression of neural phenotypes and secretion of neurotrophic factors. Acta Biomater 10:4425鈥?436CrossRef
  Mahmoud KA, Mena JA, Male KB, Hrapovic S, Kamen A, Luong JHT (2010) Effect of surface charge on the cellular uptake and cytotoxicity of fluorescent labeled cellulose nanocrystals. ACS Appl Mater Int 2:2924鈥?932CrossRef
  Nel A, Xia T, Madler L, Li N (2006) Toxic potential of materials at the nanolevel. Science 311:622鈥?27CrossRef
  Ripp S (2011) Nanotoxicology in the microbial world. In: Ripp S, Henry TB (eds) Biotechnology and nanotechnology risk assessment: minding and managing the potential threats around us. American Chemical Society, Washington, pp 121鈥?40CrossRef
  Roslev P, Lentz T, Hesselsoe M (2015) Microbial toxicity of methyl tert-butyl ether (MTBE) determined with fluorescent and luminescent bioassays. Chemosphere 120:284鈥?91CrossRef
  Shi W, Menn FM, Xu T, Zhuang ZT, Beasley C, Ripp S, Zhuang J, Layton AC, Sayler GS (2014) C60 reduces the bioavailability of mercury in aqueous solutions. Chemosphere 95:324鈥?28CrossRef
  Xu T, Close DM, Sayler GS, Ripp S (2013) Genetically modified whole-cell bioreporters for environmental assessment. Ecol Indic 28:125鈥?41CrossRef
  Yu H, Yan C, Lei X, Qin Z, Yao J (2014) Novel approach to extract thermally stable cellulose nanospheres with high yield. Mater Lett 131:12鈥?5CrossRef
  
• 作者单位：Liyu Du (1) (2)
  Kelly Arnholt (2)
  Steven Ripp (2)
  Gary Sayler (2)
  Siqun Wang (3)
  Chenghua Liang (1)
  Jingkuan Wang (1)
  Jie Zhuang (2) (4)
  
  1. College of Land and Environment, Shenyang Agriculture University, Shenyang, 110866, China
  2. The Center for Environmental Biotechnology, The University of Tennessee, Knoxville, TN, 37996, USA
  3. The Center for Renewable Carbon, The University of Tennessee, Knoxville, TN, 37996, USA
  4. Key Laboratory of Pollution Ecology and Environmental Engineering, Chinese Academy of Sciences, Shenyang, 110016, China
  
• 刊物类别：Earth and Environmental Science
• 刊物主题：Environment
  Environment
  Monitoring, Environmental Analysis and Environmental Ecotoxicology
  Ecology
  Environmental Management
  
• 出版者：Springer Netherlands
• ISSN：1573-3017

文摘

The aim of this study was to evaluate the biological toxicity of cellulose nanocrystals (CNCs) using the constitutively bioluminescent luxCDABE-based bioreporter Escherichia coli 652T7. The effects of CNCs on E. c oli 652T7 biotoxicity were investigated at different CNC concentrations, reaction times, and IC₅₀ values. CNC toxicity was also compared with and without ultrasonic dispersion to establish dispersibility effects. The results demonstrated that CNCs were not significantly toxic at concentrations at or below 250 mg/L. At concentrations higher than 300 mg/L, toxicity increased linearly as CNC concentrations increased up to 2000 mg/L. IC₅₀ calculations demonstrated an increase in cytotoxicity as CNC exposure times increased, and elevated dispersibility of the CNCs were shown to increase cytotoxicity effects. These results suggest that CNCs can impact microbial populations if elevated concentration thresholds are met. Keywords Cellulose nanocrystals Toxicity Lux Bioreporter Bioluminescence