Standardized application of yeast bioluminescent reporters as endocrine disruptor screen for comparative analysis of wastewater effluents from membrane bioreactor and traditional activated sludge

详细信息    查看全文

• 作者：Jun Wang ; Melanie Eldridge ; Fu-min Menn ; Todd Dykes ; Gary Sayler
• 关键词：Wastewater treatment ; Endocrine disruptor ; Yeast bioluminescent reporter ; BLYES ; Traditional activated sludge ; Membrane bioreactor
• 刊名：Ecotoxicology
• 出版年：2015
• 出版时间：December 2015
• 年：2015
• 卷：24
• 期：10
• 页码：2088-2099
• 全文大小：1,028 KB
• 参考文献：Chang H-S, Choo K-H, Lee B, Choi S-J (2009) The methods of identification, analysis, and removal of endocrine disrupting compounds (EDCs) in water. J Hazard Mater 172:1-2CrossRef
  Clara M, Kreuzinger N, Strenn B, Gans O, Kroiss H (2005a) The solids retention time-a suitable design parameter to evaluate the capacity of wastewater treatment plants to remove micropollutants. Water Res 39:97-06. doi:10.-016/?j.?watres.-004.-8.-36 CrossRef
  Clara M, Strenn B, Gans O, Martinez E, Kreuzinger N, Kroiss H (2005b) Removal of selected pharmaceuticals, fragrances and endocrine disrupting compounds in a membrane bioreactor and conventional wastewater treatment plants. Water Res 39:4797-807CrossRef
  Di Dea Bergamasco AM et al (2011) Bioluminescent yeast estrogen assay (BLYES) as a sensitive tool to monitor surface and drinking water for estrogenicity. J Environ Monit 13:3288-293. doi:10.-039/?C1EM10464K CrossRef
  Drewes JE, Hemming J, Schauer JJ, Sonzogni W (2006) Removal of endocrine disrupting compounds in water reclamation processes. Water Environment Research Foundation: Wastewater Treatment and Reuse, Final Report
  Eldridge ML, Sanseverino J, Layton AC, Easter JP, Schultz TW, Sayler GS (2007) Saccharomyces cerevisiae BLYAS, a new bioluminescent bioreporter for detection of androgenic compounds. Appl Environ Microbiol 73:6012-018CrossRef
  Frye C et al (2012) Endocrine disrupters: a review of some sources, effects, and mechanisms of actions on behaviour and neuroendocrine systems. J Neuroendocrinol 24:144-59. doi:10.-111/?j.-365-2826.-011.-2229.?x CrossRef
  Gaulke LS, Strand SE, Kalhorn TF, Stensel HD (2009) Estrogen biodegradation kinetics and estrogenic activity reduction for two biological wastewater treatment methods. Environ Sci Technol 43:7111-116CrossRef
  Hashimoto T, Murakami T (2009) Removal and degradation characteristics of natural and synthetic estrogens by activated sludge in batch experiments. Water Res 43:573-82. doi:10.-016/?j.?watres.-008.-0.-51 CrossRef
  Holbrook RD, Novak JT, Grizzard TJ, Love NG (2002) Estrogen receptor agonist fate during wastewater and biosolids treatment processes: a mass balance analysis. Environ Sci Technol 36:4533-539CrossRef
  Hu J, Chen X, Tao G, Kekred K (2007) Fate of endocrine disrupting compounds in membrane bioreactor systems. Environ Sci Technol 41:4097-102CrossRef
  Judd S (2008) The status of membrane bioreactor technology. Trends Biotechnol 26:109-16CrossRef
  Kabir ER, Rahman MS, Rahman I (2015) A review on endocrine disruptors and their possible impacts on human health. Environ Toxicol Pharmacol 40:241-58. doi:10.-016/?j.?etap.-015.-6.-09 CrossRef
  Kim SD, Cho J, Kim IS, Vanderford BJ, Snyder SA (2007) Occurrence and removal of pharmaceuticals and endocrine disruptors in South Korean surface, drinking, and waste waters. Water Res 41:1013-021CrossRef
  Kimura K, Hara H, Watanabe Y (2007) Elimination of selected acidic pharmaceuticals from municipal wastewater by an activated sludge system and membrane bioreactors. Environ Sci Technol 41:3708-714. doi:10.-021/?es061684z CrossRef
  Kirk LA, Tyler CR, Lye CM, Sumpter JP (2002) Changes in estrogenic and androgenic activities at different stages of treatment in wastewater treatment works. Environ Toxicol Chem 21:972-79CrossRef
  Kolpin DW, Furlong ET, Meyer MT, Thurman EM, Zaugg SD, Barber LB, Buxton HT (2002) Pharmaceuticals, hormones, and other organic wastewater contaminants in US streams, 1999-000: a national reconnaissance. Environ Sci Technol 36:1202-211CrossRef
  Krifaton C et al (2013) Application of a yeast estrogen reporter system for screening zearalenone degrading microbes. J Hazard Mater 244:429-35CrossRef
  Lee DG, Zhao F, Rezenom YH, Russell DH, Chu K-H (2012) Biodegradation of triclosan by a wastewater microorganism. Water Res 46:4226-234CrossRef
  Liu Z-h, Kanjo Y, Mizutani S (2009) Removal mechanisms for endocrine disrupting compounds (EDCs) in wastewater treatment—physical means, biodegradation, and chemical advanced oxidation: a review. Sci Total Environ 407:731-48. doi:10.-016/?j.?scitotenv.-008.-8.-39 CrossRef
  Luo Y et al (2014) A review on the occurrence of micropollutants in the aquatic environment and their fate and removal during wastewater treatment. Sci Total Environ 473:619-41CrossRef
  Martin OV, Lai KM, Scrimshaw MD, Lester JN (2005) Receiver operating characteristic analysis for environmental diagnosis. A potential application to endocrine disruptor screening: in vitro estrogenicity bioassays. Environ Sci Technol 39:5349-355. doi:10.-021/?es048598o CrossRef
  Murk AJ et al (2002) Detection of estrogenic potency in wastewater and surface water with three in vitro bioassays. Environ Toxicol Chem 21:16-3CrossRef
  Osimitz TG et al (2012) Lack of androgenicity and estrogenicity of the three monomers used in Eastman’s Tritan?copolyesters. Food Chem Toxicol 50:2196-205CrossRef
  Purvis IJ
• 作者单位：Jun Wang (1)
  Melanie Eldridge (2)
  Fu-min Menn (1)
  Todd Dykes (3)
  Gary Sayler (1)
  
  1. Center for Environmental Biotechnology, University of Tennessee, Knoxville, TN, 37996, USA
  2. Department of Biology and Environmental Science, University of New Haven, West Haven, CT, 06516, USA
  3. Hallsdale-Powell Utility District, Powell, TN, 37849, USA
  
• 刊物类别：Earth and Environmental Science
• 刊物主题：Environment
  Environment
  Monitoring, Environmental Analysis and Environmental Ecotoxicology
  Ecology
  Environmental Management
  
• 出版者：Springer Netherlands
• ISSN：1573-3017

文摘

A standardized protocol is demonstrated for bioluminescent strains Saccharomyces cerevisiae BLYES, BLYAS and BLYR as high-throughput screening tools to monitor the estrogenic, androgenic and toxic potencies in wastewater. The sensitivity and reproducibility of the assay in wastewater monitoring was evaluated for 7 day semi-continuous batch reactor using activated sludge with hormones spiked raw sewage. Yeast bioluminescent assay successfully captured the rapid removal of estrogenic and androgenic activities in the bioreactors, and demonstrated rapid response (? h) with good reproducibility. This standardized protocol was then applied in a 12 months monitoring of the effluent of a WWTP located at Powell, TN, USA featuring parallel-operated full-scale membrane bioreactor (MBR) and traditional activated sludge (TAS) treatment. Monitoring results showed that estrogenic activity was persistent in all TAS and most MBR effluent samples, while residual androgenic activity was non-detectable throughout the monitored period. The estrogenic equivalents (EEQ) in TAS effluent ranged from 21.61 ng/L to 0.04 pg/L and averaged 3.25 ng/L. The EEQ in MBR effluent ranged from 2.88 ng/L to 0.0134 pg/L and averaged ~10 fold less (0.32 ng/L) than TAS. Despite the large temporal variation, MBR effluent EEQ was consistently lower than TAS on any given sampling date. Most MBR effluent samples also exhibited less cytotoxicity than TAS. Further analysis did not demonstrate significant correlation between effluent EEQ level and WWTP operational parameters including MLSS, SRT, HRT and BOD. Keywords Wastewater treatment Endocrine disruptor Yeast bioluminescent reporter BLYES Traditional activated sludge Membrane bioreactor