Assignments of human integrin α1I domain in the apo and Mg2+ bound states

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• 作者：Yanni K.-Y. Chin (1)
  Stephen Headey (1)
  Biswaranjan Mohanty (1) (3)
  Jonas Emsley (2)
  Jamie S. Simpson (1)
  Martin J. Scanlon (1) (3)
  
• 关键词：Integrin ; Integrin αI domain ; Collagen ; Metal ion dependent adhesion site ; Magnesium ion
• 刊名：Biomolecular NMR Assignments
• 出版年：2014
• 出版时间：April 2014
• 年：2014
• 卷：8
• 期：1
• 页码：117-121
• 全文大小：1,869 KB
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• 作者单位：Yanni K.-Y. Chin (1)
  Stephen Headey (1)
  Biswaranjan Mohanty (1) (3)
  Jonas Emsley (2)
  Jamie S. Simpson (1)
  Martin J. Scanlon (1) (3)
  
  1. Medicinal Chemistry, Monash Institute of Pharmaceutical Sciences, Monash University (Parkville Campus), 381 Royal Parade, Parkville, VIC, 3052, Australia
  3. ARC Centre of Excellence for Coherent X-ray Science, Monash University, Parkville, Australia
  2. Centre for Biomolecular Sciences, School of Pharmacy, University of Nottingham, University Park, Nottingham, NG7 2RD, UK
  
• ISSN：1874-270X

文摘

The α1β1 integrin receptor binds to its main extracellular ligand, collagen, through an inserted domain in its α-subunit called the αI domain (αI). αI contains a metal binding site that allows collagen to coordinate to the domain through a divalent metal ion. Here we report the backbone assignments of the apo and Mg2+ bound state of the isolated human α1I and the chemical shift changes resulting from metal coordination.